Effective characterization of Salmonella Enteritidis by most probable number (MPN) followed by multiplex polymerase chain reaction (PCR) methods

Nontyphoidal Salmonella (NTS) is a relevant pathogen involved in gastroenteritis outbreaks worldwide. In this study, we determined the capacity to combine the most probable number (MPN) and multiplex polymerase chain reaction (PCR) methods to characterize the most important Salmonella serotypes in r...

Full description

Saved in:
Bibliographic Details
Published in:Environmental science and pollution research international 2017-02, Vol.24 (5), p.4828-4834
Main Authors: Zappelini, Lincohn, Martone-Rocha, Solange, Dropa, Milena, Matté, Maria Helena, Tiba, Monique Ribeiro, Breternitz, Bruna Suellen, Razzolini, Maria Tereza Pepe
Format: Article
Language:English
Subjects:
Aquatic Pollution
Atmospheric Protection/Air Quality Control/Air Pollution
Banding
Earth and Environmental Science
Ecotoxicology
Environment
Environmental Chemistry
Environmental Health
Environmental science
Gastroenteritis
Microorganisms
Most probable number
Multiplex Polymerase Chain Reaction
Multiplexing
Outbreaks
Polymerase chain reaction
Probability
Raw sewage
Research Article
Salmonella
Salmonella enteritidis
Salmonella enteritidis - genetics
Salmonella enteritidis - isolation & purification
Salmonella typhimurium
Serotypes
Serotyping
Sewage
Sewage disposal
Waste Water Technology
Water Management
Water Pollution Control
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Nontyphoidal Salmonella (NTS) is a relevant pathogen involved in gastroenteritis outbreaks worldwide. In this study, we determined the capacity to combine the most probable number (MPN) and multiplex polymerase chain reaction (PCR) methods to characterize the most important Salmonella serotypes in raw sewage. A total of 499 isolates were recovered from 27 raw sewage samples and screened using two previously described multiplex PCR methods. From those, 123 isolates were selected based on PCR banding pattern—identical or similar to Salmonella Enteritidis and Salmonella Typhimurium—and submitted to conventional serotyping. Results showed that both PCR assays correctly serotyped Salmonella Enteritidis, however, they presented ambiguous results for Salmonella Typhimurium identification. These data highlight that MPN and multiplex PCR can be useful methods to describe microbial quality in raw sewage and suggest two new PCR patterns for Salmonella Enteritidis identification.
ISSN:0944-1344
1614-7499
DOI:10.1007/s11356-016-8109-8