A Novel Wheat Nicotianamine Synthase Gene, TaNAS-D, Confers High Salt Tolerance in Transgenic Arabidopsis

Nicotianamine synthase (NAS) plays a pivotal role in balancing the concentrations of heavy metals in plants, but its characteristics and functions in salt stress responses are not completely understood, particularly in wheat. In this study, the salt-induced gene TaNAS-D was cloned from wheat and cha...

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Bibliographic Details
Published in:Plant molecular biology reporter 2017-04, Vol.35 (2), p.252-264
Main Authors: Han, Jie, Zhang, Wei, Sun, Lijing, Su, Qiannan, Li, Zijing, Fan, Xiaoli, Zhang, Na, Pan, Ruiqing, Cui, Fa, Ji, Jun, Li, Hui, Li, Junming
Format: Article
Language:English
Subjects:
Arabidopsis
Bioinformatics
Biomedical and Life Sciences
Heavy metals
Hydrogen peroxide
Life Sciences
Metabolomics
Metal concentrations
Original Paper
Plant biology
Plant Breeding/Biotechnology
Plant Sciences
Proteomics
Salt tolerance
Sodium chloride
Triticum aestivum
Wheat
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Summary:Nicotianamine synthase (NAS) plays a pivotal role in balancing the concentrations of heavy metals in plants, but its characteristics and functions in salt stress responses are not completely understood, particularly in wheat. In this study, the salt-induced gene TaNAS-D was cloned from wheat and characterized. TaNAS-D , localized throughout the cell, is mainly expressed in developed vascular bundle tissues and is responsive to NaCl, ABA, and H 2 O 2 stresses. Overexpression of TaNAS-D in Arabidopsis led to elevated NA levels and enhanced salt stress tolerance, which was demonstrated by higher germination rates and improved growth of TaNAS-D transgenic Arabidopsis plants compared with WT when exposed to salt stress. Further investigation revealed that TaNAS-D transgenic Arabidopsis plants displayed higher K + /Na + ratios, lower malondialdehyde (MDA) levels, and less ion leakage (IL) consistently accompanied by increased peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) activities, thereby reducing membrane injury. Moreover, TaNAS-D overexpression under salt stress increased AtSOS1 , AtSOS2 , AtSOS3 , AtFAD5 , and AtSAD1 transcript levels. These findings indicate that TaNAS-D plays a positive role in salt tolerance by improving the antioxidant defense system and upregulating salt overly sensitive (SOS) pathway genes.
ISSN:0735-9640
1572-9818
DOI:10.1007/s11105-016-1018-7