Identification and characterization of a library of surfactins and fengycins from a marine endophytic Bacillus sp

An endophytic bacterial strain from a marine green alga, Ulva lactuca, was isolated and identified by 16S rRNA gene sequencing method. The bacterial isolate was found to secrete two major families of cyclic depsilipopeptides, surfactins, and fengycins. Sequencing of the isolated lipopeptides was car...

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Bibliographic Details
Published in:Journal of basic microbiology 2016-11, Vol.56 (11), p.1159-1172
Main Authors: Nair, Divya, Vanuopadath, Muralidharan, Nair, Bipin G., Pai, Jayashree Gopalakrishna, Nair, Sudarslal Sadasivan
Format: Article
Language:English
Subjects:
Bacillus
Bacillus - genetics
Bacillus - isolation & purification
Bacillus - metabolism
Chromatography, High Pressure Liquid
Collision-induced dissociation
Cyclic depsipeptide
Cyclic lipopeptide
Electron transfer dissociation
Endophytes - isolation & purification
Gene Library
Genes, rRNA
Lipopeptides - chemistry
Lipopeptides - isolation & purification
Lipopeptides - metabolism
Lipopeptides - pharmacology
Marine endophyte
Mass spectrometry
Oryza - drug effects
Oryza - growth & development
Oryza sativa
Peptides, Cyclic - chemistry
Peptides, Cyclic - isolation & purification
Peptides, Cyclic - metabolism
Peptides, Cyclic - pharmacology
RNA, Ribosomal, 16S
Spectrometry, Mass, Electrospray Ionization
Surface-Active Agents - chemistry
Surface-Active Agents - isolation & purification
Ulva - microbiology
Ulva lactuca
Vigna - drug effects
Vigna - growth & development
Vigna radiata
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Summary:An endophytic bacterial strain from a marine green alga, Ulva lactuca, was isolated and identified by 16S rRNA gene sequencing method. The bacterial isolate was found to secrete two major families of cyclic depsilipopeptides, surfactins, and fengycins. Sequencing of the isolated lipopeptides was carried out using the MSn data obtained from an electrospray ionization (ESI) ion trap mass spectrometer coupled to an HPLC system. The assigned sequences were confirmed by a chemical derivatization approach involving esterification followed by mass spectrometric analysis. Distinction of leucine residues from isoleucine was established through a combined electron transfer dissociation‐collision‐induced dissociation (ETD‐CID) method. The fengycins described in this study were found to cause significant delay of growth of two plants, Vigna radiata (mung bean) and Oryza sativa (rice). To the best of our knowledge, this is the first study describing identification and characterization of cyclic peptides from an endophytic Bacillus sp. isolated from marine algae.
ISSN:0233-111X
1521-4028
DOI:10.1002/jobm.201600029