Characterization and complete genome sequence of a panicovirus from Bermuda grass by high-throughput sequencing

Bermuda grass samples were examined by transmission electron microscopy and 28-30 nm spherical virus particles were observed. Total RNA from these plants was subjected to high-throughput sequencing (HTS). The nearly full genome sequence of a panicovirus was identified from one HTS scaffold. Sanger s...

Full description

Saved in:
Bibliographic Details
Published in:Archives of virology 2017-04, Vol.162 (4), p.1099-1102
Main Authors: Tahir, Muhammad N., Lockhart, Ben, Grinstead, Samuel, Mollov, Dimitre
Format: Article
Language:English
Subjects:
Amino acids
Annotated Sequence Record
Asymptomatic
Base Sequence
Biomedical and Life Sciences
Biomedicine
Cloning
Genome, Viral
Genomes
High-Throughput Nucleotide Sequencing
Infectious Diseases
Medical Microbiology
Molecular Sequence Data
Molecular weight
Open Reading Frames
Phylogenetics
Phylogeny
Plant Diseases - virology
Poaceae - virology
Proteins
RNA polymerase
RNA, Viral
Tombusviridae
Tombusviridae - classification
Tombusviridae - genetics
Tombusviridae - isolation & purification
Transmission electron microscopy
Viral Proteins - genetics
Virology
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bermuda grass samples were examined by transmission electron microscopy and 28-30 nm spherical virus particles were observed. Total RNA from these plants was subjected to high-throughput sequencing (HTS). The nearly full genome sequence of a panicovirus was identified from one HTS scaffold. Sanger sequencing was used to confirm the HTS results and complete the genome sequence of 4404 nt. This virus was provisionally named Bermuda grass latent virus (BGLV). Its predicted open reading frames follow the typical arrangement of the genus Panicovirus . Based on sequence comparisons and phylogenetic analyses BGLV differs from other viruses and therefore taxonomically it is a new member of the genus Panicovirus , family Tombusviridae .
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-016-3165-4