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Galactomannan from Schizolobium amazonicum seed and its sulfated derivatives impair metabolism in HepG2 cells
This study evaluated the effects of native galactomannan from Schizolobium amazonicum seeds and its sulfated forms on certain metabolic parameters of HepG2 cells. Aqueous extraction from S. amazonicum seeds furnished galactomannan with 3.2:1 Man:Gal ratio (SAGM) and molar mass of 4.34×105g/mol. The...
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Published in: | International journal of biological macromolecules 2017-08, Vol.101, p.464-473 |
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description | This study evaluated the effects of native galactomannan from Schizolobium amazonicum seeds and its sulfated forms on certain metabolic parameters of HepG2 cells. Aqueous extraction from S. amazonicum seeds furnished galactomannan with 3.2:1 Man:Gal ratio (SAGM) and molar mass of 4.34×105g/mol. The SAGM fraction was subjected to sulfation using chlorosulfonic acid to obtain SAGMS1 and SAGMS2 with DS of 0.4 and 0.6, respectively. Cytotoxicity of SAGM, SAGMS1, and SAGMS2 was evaluated in human hepatocellular carcinoma cells (HepG2). After 72h, SAGM decreased the viability of HepG2 cells by 50% at 250μg/mL, while SAGMS1 reduced it by 30% at the same concentration. SAGM, SAGMS1, and SAGMS2 promoted a reduction in oxygen consumption and an increase in lactate production in non-permeabilized HepG2 cells after 72h of treatment. These results suggest that SAGM, SAGMS1, and SAGMS2 could be recognized by HepG2 cells and might trigger alterations that impair its survival. These effects could be implicated in the modification of the oxidative phosphorylation process in HepG2 cells and activation of the glycolytic pathway. |
doi_str_mv | 10.1016/j.ijbiomac.2017.03.090 |
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Aqueous extraction from S. amazonicum seeds furnished galactomannan with 3.2:1 Man:Gal ratio (SAGM) and molar mass of 4.34×105g/mol. The SAGM fraction was subjected to sulfation using chlorosulfonic acid to obtain SAGMS1 and SAGMS2 with DS of 0.4 and 0.6, respectively. Cytotoxicity of SAGM, SAGMS1, and SAGMS2 was evaluated in human hepatocellular carcinoma cells (HepG2). After 72h, SAGM decreased the viability of HepG2 cells by 50% at 250μg/mL, while SAGMS1 reduced it by 30% at the same concentration. SAGM, SAGMS1, and SAGMS2 promoted a reduction in oxygen consumption and an increase in lactate production in non-permeabilized HepG2 cells after 72h of treatment. These results suggest that SAGM, SAGMS1, and SAGMS2 could be recognized by HepG2 cells and might trigger alterations that impair its survival. These effects could be implicated in the modification of the oxidative phosphorylation process in HepG2 cells and activation of the glycolytic pathway.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2017.03.090</identifier><identifier>PMID: 28347788</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Antineoplastic Agents - chemistry ; Antineoplastic Agents - pharmacology ; Cell Respiration - drug effects ; Cell Survival - drug effects ; Fabaceae - chemistry ; Galactomannans ; Hep G2 Cells ; HepG2 cells ; Humans ; Lactic Acid - metabolism ; Mannans - chemistry ; Mannans - pharmacology ; Oxidative Phosphorylation - drug effects ; Pyruvic Acid - metabolism ; Seeds - chemistry ; Sulfates - chemistry ; Sulfation</subject><ispartof>International journal of biological macromolecules, 2017-08, Vol.101, p.464-473</ispartof><rights>2017 Elsevier B.V.</rights><rights>Copyright © 2017 Elsevier B.V. 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These effects could be implicated in the modification of the oxidative phosphorylation process in HepG2 cells and activation of the glycolytic pathway.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>28347788</pmid><doi>10.1016/j.ijbiomac.2017.03.090</doi><tpages>10</tpages></addata></record> |
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subjects | Antineoplastic Agents - chemistry Antineoplastic Agents - pharmacology Cell Respiration - drug effects Cell Survival - drug effects Fabaceae - chemistry Galactomannans Hep G2 Cells HepG2 cells Humans Lactic Acid - metabolism Mannans - chemistry Mannans - pharmacology Oxidative Phosphorylation - drug effects Pyruvic Acid - metabolism Seeds - chemistry Sulfates - chemistry Sulfation |
title | Galactomannan from Schizolobium amazonicum seed and its sulfated derivatives impair metabolism in HepG2 cells |
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