Direct labeling of serum proteins by fluorescent dye for antibody microarray

Analysis of serum proteome by antibody microarray is used to identify novel biomarkers and to study signaling pathways including protein phosphorylation and protein-protein interactions. Labeling of serum proteins is important for optimal performance of the antibody microarray. Proper choice of fluo...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and biophysical research communications 2017-05, Vol.486 (3), p.824-826
Main Authors: Klimushina, M.V., Gumanova, N.G., Metelskaya, V.A.
Format: Article
Language:English
Subjects:
Antibodies - chemistry
Antibody
Blood Proteins - chemistry
Blood Proteins - isolation & purification
Carbocyanines - chemistry
Fluorescent Dyes - chemistry
Fluorophore
Humans
Microarray
Protein Array Analysis - instrumentation
Protein Array Analysis - methods
Protein labeling
Staining and Labeling - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Analysis of serum proteome by antibody microarray is used to identify novel biomarkers and to study signaling pathways including protein phosphorylation and protein-protein interactions. Labeling of serum proteins is important for optimal performance of the antibody microarray. Proper choice of fluorescent label and optimal concentration of protein loaded on the microarray ensure good quality of imaging that can be reliably scanned and processed by the software. We have optimized direct serum protein labeling using fluorescent dye Arrayit Green 540 (Arrayit Corporation, USA) for antibody microarray. Optimized procedure produces high quality images that can be readily scanned and used for statistical analysis of protein composition of the serum.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2017.03.136