Sensitivity of tumor surface brushings to detect human papilloma virus DNA in head and neck cancer

Highlights • HPV brush test from the tumor surface was compared with HPV DNA isolation from formalin-fixed and paraffin-embedded (FFPE) from tumor biopsies. • Brushings from tumor surfaces had a sensitivity of 83% (95%CI: 67–92%) and specificity of 85% (95%CI: 72–93%) when compared with FFPE derived...

Full description

Saved in:
Bibliographic Details
Published in:Oral oncology 2017-04, Vol.67, p.103-108
Main Authors: Kofler, Barbara, Borena, Wegene, Manzl, Claudia, Dudas, Jozsef, Wegscheider, Anne-Sophie, Jansen-Dürr, Pidder, Schartinger, Volker, Riechelmann, Herbert
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Brush test
DNA, Viral - analysis
Female
Head and neck cancer
Head and Neck Neoplasms - virology
Hematology, Oncology and Palliative Medicine
Human papillomavirus
Humans
Limit of Detection
Male
Middle Aged
Otolaryngology
p16
Papillomaviridae - genetics
Papillomaviridae - isolation & purification
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Highlights • HPV brush test from the tumor surface was compared with HPV DNA isolation from formalin-fixed and paraffin-embedded (FFPE) from tumor biopsies. • Brushings from tumor surfaces had a sensitivity of 83% (95%CI: 67–92%) and specificity of 85% (95%CI: 72–93%) when compared with FFPE derived viral DNA determination. • P16-IHC sensitivity was 78% (95% CI = 62–89%) and specificity was 79% (95% CI = 66–89%) compared with the FFPE reference.
ISSN:1368-8375
1879-0593
DOI:10.1016/j.oraloncology.2017.02.013