A novel thermally stable heteropolysaccharide-based bioflocculant from hydrocarbonoclastic strain Kocuria rosea BU22S and its application in dye removal

A new bioflocculant named pKr produced by hydrocarbonoclastic strain Kocuria rosea BU22S (KC152976) was investigated. Gas chromatography-flame ionization detector (GC-FID) analysis confirmed the high potential of the strain BU22S in the degradation of n-alkanes. Plackett-Burman experimental design a...

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Bibliographic Details
Published in:Environmental technology 2018-04, Vol.39 (7), p.859-872
Main Authors: Chouchane, Habib, Mahjoubi, Mouna, Ettoumi, Besma, Neifar, Mohamed, Cherif, Ameur
Format: Article
Language:English
Subjects:
Alkanes
bioflocculant
Bioremediation
Chemical analysis
Chromatography
Color removal
crude oil degradation
Decoloring
Decolorization
dye removal
Dyes
Experimental design
Flame ionization
Flame ionization detectors
Flocculation
flocculation activity
Fourier transforms
Functional groups
Galactose
Gas chromatography
Glucose
Infrared analysis
Ionization
Ionization counters
Kaolin
Kocuria rosea BU22S
Peptones
Polysaccharides
Proteins
Response surface methodology
Saccharides
Thermal stability
Thin layer chromatography
Uronic acid
Xylose
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Summary:A new bioflocculant named pKr produced by hydrocarbonoclastic strain Kocuria rosea BU22S (KC152976) was investigated. Gas chromatography-flame ionization detector (GC-FID) analysis confirmed the high potential of the strain BU22S in the degradation of n-alkanes. Plackett-Burman experimental design and response surface methodology were carried out to optimize pKr production. Glucose, peptone and incubation time were found to be the most significant factors affecting bioflocculant production. Maximum pKr production was about 4.72 ± 0.02 g/L achieved with 15.61 g/L glucose, 6.45 g/L peptone and 3 days incubation time. Chemical analysis of pKr indicated that it contained 71.62% polysaccharides, 16.36% uronic acid and 2.83% proteins. Thin layer chromatography analysis showed that polysaccharides fraction consisted of galactose and xylose. Fourier transform infrared analysis revealed the presence of many functional groups, hydroxyl, carboxyl, methoxyl, acetyl and amide that likely contribute to flocculation. K. rosea pKr showed high flocculant potential using kaolin clay at different pH (2-11), temperature (0-100°C) and cation concentrations. The bioflocculant was particularly effective in flocculating soluble anionic dyes, Reactive Blue 4 and Acid Yellow, with a decolorization efficiency of 76.4% and 72.6%, respectively. The outstanding flocculating performances suggest that pKr could be useful for bioremediation applications.
ISSN:0959-3330
1479-487X
DOI:10.1080/09593330.2017.1313886