The impact of cyanoglycoside rich fraction isolated from Cassava ( Manihot esculenta) on alcohol induced oxidative stress

• Published in: Toxicon (Oxford) 2003-09, Vol.42 (4), p.367-372
• Main Authors: Boby, R.G, Indira, M
• Format: Article
• Language: English
• Subjects: Alcohol; Animals; Antioxidants - pharmacology; Cassava; Central Nervous System Depressants - pharmacology; Cyanoglycosides; Drug Interactions; Ethanol - pharmacology; Glycosides - pharmacology; Lipid peroxidation; Lipid Peroxidation - drug effects; Lipid Peroxides - metabolism; Liver - drug effects; Liver - metabolism; Male; Malondialdehyde; Malondialdehyde - metabolism; Manihot - chemistry; Manihot - metabolism; Oxidative Stress - drug effects; Plant Extracts - pharmacology; Rats; Rats, Sprague-Dawley; Superoxide dismutase
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/S0041-0101(03)00165-X

The effects of feeding a cassava ( Manihot esculenta) rich diet on alcohol induced peroxidative damages were investigated in male albino rats. Rats were divided into four groups and maintained for 60 days as follows. (1) Control group: cassava free diet, (2) alcohol group: cassava free diet+ethanol (4 g/kg body wt/day), (3) cassava group: cassava diet and (4) alcohol+cassava group: cassava diet+ethanol (4 g/kg body wt/day). Results revealed that alcohol induced significant lipid peroxidation, since the lipid peroxidation products malondialdehyde (MDA), hydroperoxides and conjugated dienes were elevated in the liver. The activities of free radical scavenging enzymes such as superoxide dismutase (SOD), catalase and glutathione reductase were reduced and glutathione content was decreased in the liver. But the co-administration of a cassava rich diet increased the activity of free radical scavenging enzymes and glutathione content. The level of lipid peroxides in the liver was also decreased on co-administration of cassava. But the oxidative damage caused by cassava was potentiated by alcohol administration. These studies suggested that consumption of alcohol along with cassava offered some protection against the alcohol induced oxidative stress. So we isolated the cyanoglycoside rich fraction from cassava and its impact on rats administered alcohol was also investigated. The results revealed that alcohol induced oxidative stress was potentiated by the co-administration of cyanoglycoside rich fraction. These studies suggested that the fiber and antioxidant vitamins present in the cassava may be playing a protective role against the alcohol induced oxidative stress.