Production of exopolysaccharides by selected Bacillus strains: Optimization of media composition to maximize the yield and structural characterization

Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast...

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Bibliographic Details
Published in:International journal of biological macromolecules 2017-09, Vol.102, p.539-549
Main Authors: Malick, Afshan, Khodaei, Nastaran, Benkerroum, Noreddine, Karboune, Salwa
Format: Article
Language:English
Subjects:
Bacillus - growth & development
Bacillus - metabolism
Bacillus spp
Bioreactors - microbiology
Culture Media - chemistry
Exopolysaccharides
Fermentation
Heteropolymers
Medium composition
Molecular Weight
Monosaccharides - analysis
Optimization
Polysaccharides, Bacterial - biosynthesis
Polysaccharides, Bacterial - chemistry
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Summary:Selected Bacillus strains were investigated for the production of exopolysaccharides (EPS) by fermentation in three different culture media: (i) mineral base-medium with added yeast extract (M1), (ii) succinate-containing mineral base-medium with added yeast extract (M2) and (iii) tryptone and yeast extract-containing base-medium (M3). Modest EPS yield of 6.7g/L was recorded for Bacillus amyloliquefaciens 23350 grown on M1, where the EPS produced was characterized by a low MW (
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2017.03.151