Measurement of Pro-Islet Amyloid Polypeptide (1-48) in Diabetes and Islet Transplants

Islet amyloid is a feature of β-cell failure in type 2 diabetes (T2D) and type 1 diabetes (T1D) recipients of islet transplants. Islet amyloid contains islet amyloid polypeptide (IAPP; amylin), a circulating peptide that is produced in β cells by processing of its precursor, proIAPP1-67, via an inte...

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Published in:The journal of clinical endocrinology and metabolism 2017-07, Vol.102 (7), p.2595-2603
Main Authors: Courtade, Jaques A, Klimek-Abercrombie, Agnieszka M, Chen, Yi-Chun, Patel, Nirja, Lu, Phoebe Y T, Speake, Cate, Orban, Paul C, Najafian, Behzad, Meneilly, Graydon, Greenbaum, Carla J, Warnock, Garth L, Panagiotopoulos, Constadina, Verchere, C Bruce
Format: Article
Language:English
Subjects:
Adult
Amylin
Amyloid
Amyloid - secretion
Biomarkers - blood
Blood plasma
Case-Control Studies
Cellular stress response
Children
Cross-reactivity
Diabetes
Diabetes mellitus
Diabetes Mellitus, Type 1 - blood
Diabetes Mellitus, Type 1 - physiopathology
Diabetes Mellitus, Type 1 - surgery
Diabetes Mellitus, Type 2 - blood
Diabetes Mellitus, Type 2 - physiopathology
Diabetes Mellitus, Type 2 - surgery
Enzyme-Linked Immunosorbent Assay
Female
Follow-Up Studies
Humans
Immunoassay
Immunoreactivity
Insulin
Islet Amyloid Polypeptide - blood
Islet cells
Islets of Langerhans Transplantation
Male
Middle Aged
Pancreatic islet transplantation
Plasma
Plasma levels
Polypeptides
Proinsulin - metabolism
Reference Values
Risk Assessment
Transplantation
Transplants & implants
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Summary:Islet amyloid is a feature of β-cell failure in type 2 diabetes (T2D) and type 1 diabetes (T1D) recipients of islet transplants. Islet amyloid contains islet amyloid polypeptide (IAPP; amylin), a circulating peptide that is produced in β cells by processing of its precursor, proIAPP1-67, via an intermediate form, proIAPP1-48. Elevated proinsulin to C-peptide ratios in the plasma of persons with diabetes suggest defects in β-cell prohormone processing. Determine whether plasma levels of precursor forms of IAPP are elevated in diabetes. We developed an immunoassay to detect proIAPP1-48 in human plasma, and we determined the ratio of proIAPP1-48 to mature IAPP in subjects with T1D, T2D, recipients of islet transplants, and healthy controls. The proIAPP1-48 immunoassay had a limit of detection of 0.18 ± 0.06 pM and cross-reactivity with intact proIAPP1-67
ISSN:0021-972X
1945-7197
DOI:10.1210/jc.2016-2773