Ultrasensitive electrochemical biosensing platform based on spherical silicon dioxide/molybdenum selenide nanohybrids and triggered Hybridization Chain Reaction

• Published in: Biosensors & bioelectronics 2017-08, Vol.94, p.616-625
• Main Authors: Shuai, Hong-Lei, Wu, Xu, Huang, Ke-Jing, Zhai, Zi-Bo
• Format: Article
• Language: English
• Subjects: Binding Sites; Biosensing Techniques - methods; DNA - chemistry; DNA - isolation & purification; Electrochemical biosensor; Electrochemical Techniques - methods; Graphene oxide–gold nanoparticles; Graphite - chemistry; Humans; Hybridization Chain Reaction; Metal Nanoparticles - chemistry; Molybdenum - chemistry; Sandwich-type; Signal amplification; Silicon Dioxide - chemistry; Spherical silicon dioxide/molybdenum selenide
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2017.03.058

An ultrasensitive sandwich-type electrochemical biosensor for DNA detection is developed based on spherical silicon dioxide/molybdenum selenide (SiO2@MoSe2) and graphene oxide–gold nanoparticles (GO–AuNPs) hybrids as carrier triggered Hybridization Chain Reaction (HCR) coupling with multi-signal amplification. The proposed sensoring assay utilizes a spherical SiO2@MoSe2/AuNPs as sensing platform and GO–AuNPs hybrids as carriers to supply vast binding sites. H2O2+HQ system is used for DNA detection and HCR as the signal and selectivity enhancer. The sensor is designed in sandwich type to increase the specificity. As a result, the present biosensor exhibits a good dynamic range from 0.1fM to 100pM with a low detection limit of 0.068fM (S/N=3). This work shows a considerable potential for quantitative detection of DNA in early clinical diagnostics. •SiO2@MoSe2 was prepared with large specific surface area and good electro-conductivity.•SiO2@MoSe2 was used as sensing platform and GO–AuNPs hybrids as signal carriers.•H2O2+HQ system is used for DNA detecting with signal amplification.•The biosensor shows a detection limit of 0.068 fM and high specificity towards target DNA.