Clonal diversity of extended-spectrum beta-lactamase producing Escherichia coli isolates in fecal samples of wild animals

• Published in: FEMS microbiology letters 2017-03, Vol.364 (5)
• Main Authors: Cristóvão, Filipe, Alonso, Carla Andrea, Igrejas, Gilberto, Sousa, Margarida, Silva, Vanessa, Pereira, José Eduardo, Lozano, Carmen, Cortés-Cortés, Gerardo, Torres, Carmen, Poeta, Patrícia
• Format: Article
• Language: English
• Subjects: Animals; Animals, Wild - microbiology; Anti-Bacterial Agents - pharmacology; Bacteria; Bacterial Proteins - genetics; Bacterial Typing Techniques; beta-Lactamases - biosynthesis; Biodiversity; Coliforms; DNA Fingerprinting; E coli; Electrophoresis, Gel, Pulsed-Field; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - enzymology; Escherichia coli - genetics; Escherichia coli - isolation & purification; Feces; Feces - microbiology; Foxes - microbiology; Gel electrophoresis; Genetic Variation; Health problems; Incompatibility; Microbial Sensitivity Tests; Microbiology; Multilocus Sequence Typing; Phylogeny; Plasmids; Plasmids - classification; Polymerase Chain Reaction; Portugal - epidemiology; Pulsed-field gel electrophoresis; Replicon; Spain - epidemiology; Sus scrofa - microbiology; Wild animals; β Lactamase
• ISSN: 1574-6968; 0378-1097; 1574-6968
• DOI: 10.1093/femsle/fnx039

Abstract The clonal diversity of extended-spectrum-β-lactamase (ESBL)-producing Escherichia coli isolates from nine different species of wild animals from distinct regions of Portugal and Spain and their content in replicon plasmids were analyzed. Among the initial 53 ESBL-producing E. coli isolates that were studied (from previous studies), 28 were selected, corresponding to different animal origins with distinct ESBL types and pulsed-field gel electrophoresis (PFGE) patterns. These 28 isolates produced different ESBLs ascribed to the following families: CTX-M, SHV and TEM. The isolates were classified into three phylogenetic groups: B1 (n = 11), A (n = 10) and D (n = 7). The seven E. coli of phylogroup D were then typed by multilocus sequence typing and ascribed to four distinct sequence types: ST117, ST115, ST2001 and ST69. The clonal diversity and relationship between isolates was studied by PFGE. Lastly, the plasmids were analyzed according to their incompatibility group using the PCR-based-replicon-typing scheme. A great diversity of replicon types was identified, with up to five per isolate. Most of the CTX-M-1 and SHV-12 producing E. coli isolates carried IncI1 or IncN replicons. The diversity of ESBL-producing E. coli isolates in wild animals, which can be disseminated in the environment, emphasizes the environmental and health problems that we face nowadays. The diversity of ESBL-producing Escherichia coli isolates in wild animal, which can be disseminated in the environment, emphasizes the environmental and health problems that we face nowadays.