Impact of high hydrostatic pressure on bacterial proteostasis

• Published in: Biophysical chemistry 2017-12, Vol.231, p.3-9
• Main Authors: Gayán, Elisa, Govers, Sander K., Aertsen, Abram
• Format: Article
• Language: English
• Subjects: Bacteria - chemistry; Bacteria - growth & development; Bacteria - metabolism; Bacterial Proteins - chemistry; Bacterial Proteins - metabolism; E. coli; High hydrostatic pressure; Hydrostatic Pressure; Piezophiles; Protein Aggregates; Protein translation; Proteostasis - physiology; Resistance development
• ISSN: 0301-4622; 1873-4200
• DOI: 10.1016/j.bpc.2017.03.005

High hydrostatic pressure (HHP) is an important factor that limits microbial growth in deep-sea ecosystems to specifically adapted piezophiles. Furthermore, HHP treatment is used as a novel food preservation technique because of its ability to inactivate pathogenic and spoilage bacteria while minimizing the loss of food quality. Disruption of protein homeostasis (i.e. proteostasis) as a result of HHP-induced conformational changes in ribosomes and proteins has been considered as one of the limiting factors for both microbial growth and survival under HHP conditions. This work therefore reviews the effects of sublethal (≤100MPa) and lethal (>100MPa) pressures on protein synthesis, structure, and functionality in bacteria. Furthermore, current understanding on the mechanisms adopted by piezophiles to maintain proteostasis in HHP environments and responses developed by atmospheric-adapted bacteria to protect or restore proteostasis after HHP exposure are discussed. •Mild hydrostatic pressure reversibly impairs transcription, translation, DNA replication and cell division in E. coli.•The ability of piezophiles to grow at mild hydrostatic pressure correlates with altered ribosome and protein structures.•Heat shock proteins can support resistance against hydrostatic pressure stress.•Bacteria can evolve to acquire extreme HHP resistance