Laser and LED phototherapy on midpalatal suture after rapid maxilla expansion: Raman and histological analysis

• Published in: Lasers in medical science 2017-02, Vol.32 (2), p.263-274
• Main Authors: Rosa, Cristiane Becher, Habib, Fernando Antonio Lima, de Araújo, Telma Martins, dos Santos, Jean Nunes, Cangussu, Maria Cristina T., Barbosa, Artur Felipe Santos, de Castro, Isabele Cardoso Vieira, Pinheiro, Antônio Luiz Barbosa
• Format: Article
• Language: English
• Subjects: Animals; Biomedical materials; Bones; Collagen - metabolism; Dentistry; Formations; Histology; Inflammation - pathology; Irradiation; Lasers; Lasers, Semiconductor - therapeutic use; Light emitting diodes; Light therapy; Male; Maxilla - pathology; Maxilla - radiation effects; Medicine; Medicine & Public Health; Optical Devices; Optics; Original Article; Orthodontics; Palatal Expansion Technique; Palate - radiation effects; Photonics; Phototherapy - methods; Quantum Optics; Rats, Wistar; Rodents; Spectrum analysis; Spectrum Analysis, Raman; Spots; Sutures
• ISSN: 0268-8921; 1435-604X
• DOI: 10.1007/s10103-016-2108-3

The aim of this study was to analyze the effect of laser or LED phototherapy on the acceleration of bone formation at the midpalatal suture after rapid maxilla expansion. Forty-five rats were divided into groups at 7 days (control, expansion, expansion and laser irradiation, and expansion and LED irradiation) and into 14 days (expansion, expansion and laser in the 1st week, expansion and LED in the 1st week, expansion and laser in the 1st and 2nd weeks, expansion and LED in the 1st and 2nd weeks). Laser/LED irradiation occurred every 48 h. Expansion was accomplished with a spatula and maintained with a triple helicoid of 0.020-in stainless steel orthodontic wire. A diode laser ( λ 780 nm, 70 mW, spot of 0.04 cm 2 , t  = 257 s, SAEF of 18 J/cm 2 ) or a LED ( λ 850 ± 10 nm, 150 ± 10 mW, spot of 0.5 cm 2 , t  = 120 s, SAEF of 18 J/cm 2 ) was applied in one point in the midpalatal suture immediately behind the upper incisors. Raman spectroscopy and histological analyses of the suture region were carried and data was submitted to statistical analyses ( p  ≤ 0.05). Raman spectrum analysis demonstrated that irradiation increases hydroxyapatite in the midpalatal suture after expansion. In the histological analysis of various inflammation, there was a higher production of collagen and osteoblastic activity and less osteoclastic activity. The results showed that LED irradiation associated to rapid maxillary expansion improves bone repair and could be an alternative to the use of laser in accelerating bone formation in the midpalatal suture.