Tuning the Aggregation/Disaggregation Behavior of Graphene Quantum Dots by Structure-Switching Aptamer for High-Sensitivity Fluorescent Ochratoxin A Sensor

The design of graphene quantum dots (GQDs)-aptamer bioconjugates as the new sensing platform is very important for developing high-sensitivity fluorescent biosensors; however, achieving new bioconjugates is still a great challenge. Herein, we report the development of a new high-sensitivity fluoresc...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2017-02, Vol.89 (3), p.1704-1709
Main Authors: Wang, Song, Zhang, Yajun, Pang, Guangsheng, Zhang, Yingwei, Guo, Shaojun
Format: Article
Language:English
Subjects:
Agglomeration
Aptamers, Nucleotide - chemistry
Biosensing Techniques
Biosensors
Detection
Fluorescence
Graphene
Graphite - chemistry
Limit of Detection
Molecules
Nanoparticles
Ochratoxins - analysis
Platforms
Quantum dots
Quantum Dots - chemistry
Qunatum dots
Recovery
Sensors
Spectrometry, Fluorescence
Tuning
Wine - analysis
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Summary:The design of graphene quantum dots (GQDs)-aptamer bioconjugates as the new sensing platform is very important for developing high-sensitivity fluorescent biosensors; however, achieving new bioconjugates is still a great challenge. Herein, we report the development of a new high-sensitivity fluorescent aptasensor for the detection of ochratoxin A (OTA) based on tuning aggregation/disaggregation behavior of GQDs by structure-switching aptamers. The fluorescence sensing process for OTA detection involved two key steps: (1) cDNA-aptamer (cDNA, complementary to part of the OTA aptamer) hybridization induced the aggregation of GQD (fluorescence quenching) after cDNA was added into the GQDs-aptamer bioconjugate solution, and (2) the target of OTA triggered disaggregation of GQD aggregates (fluorescence recovery). Such new fluorescent sensing platform can be used to monitor OTA with a linear range of 0 to 1 ng/mL and very low detection limit of 13 pg/mL, which is among the best in all the developed fluorescent nanoparticles-based sensors. Such sensing strategy is also successful in analyzing OTA in practical red wine sample with 94.4–102.7% of recoveries and relative standard deviation in the range of 2.9–5.8%. The present works open a new way for signaling the target-aptamer binding event by tuning aggregation/disaggregation behavior of GQDs-bioconjugates.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.6b03913