Metabolic engineering strategies for D‐lactate over production in Escherichia coli

D‐lactate is an important chiral intermediate and a substrate for polylactic acid (PLA) production. Escherichia coli accumulate D‐lactate as the primary fermentative product, and synthesis is directly controlled by the activity of lactate dehydrogenase, the efficiency of the carbon resource utilizat...

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Bibliographic Details
Published in:Journal of chemical technology and biotechnology (1986) 2016-03, Vol.91 (3), p.576-584
Main Authors: Zhou, Li, Cui, Wen‐jing, Liu, Zhong‐mei, Zhou, Zhe‐min
Format: Article
Language:English
Subjects:
Carbon
Chromium
D-lactate production
Efficiency
Escherichia coli
fermentation
Guidelines
Lactate dehydrogenase
Manufacturing engineering
metabolic engineering
metabolic engineering strategy
polylactic acid
production costs
Strategy
yields
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Summary:D‐lactate is an important chiral intermediate and a substrate for polylactic acid (PLA) production. Escherichia coli accumulate D‐lactate as the primary fermentative product, and synthesis is directly controlled by the activity of lactate dehydrogenase, the efficiency of the carbon resource utilization, and the redox state. D‐lactate accumulation is complicated by the flux through the competing metabolic routes and the indirect regulation of energy metabolism, as well as by the unexpected interconnectivities of the cellular components in the remote metabolic routes. These effects have been extensively studied, and a number of metabolic engineering strategies have been applied to overproduce D‐lactate with high titers, yields and productivities in E. coli that have been able to reduce the production costs and precisely control the fermentation process. This review summarizes the related strategies and suggests directions for further study; these directions provide guidelines for the development of other metabolic products using E. coli as an industrial platform. © 2015 Society of Chemical Industry
ISSN:0268-2575
1097-4660
DOI:10.1002/jctb.4856