Recycling of carbon dioxide by free air CO2 enriched (FACE) Bacillus sp. SS105 for enhanced production and optimization of biosurfactant

• Published in: Bioresource technology 2017-10, Vol.242, p.2-6
• Main Authors: Maheshwari, Neha, Kumar, Madan, Thakur, Indu Shekhar, Srivastava, Shaili
• Format: Article
• Language: English
• Subjects: Bacillus sp; Biosurfactant; CO2 sequestration; Free air CO2 enrichment (FACE); Response Surface Methodology (RSM)
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2017.03.124

•Carbon concentrating Bacillus sp. was isolated from free air CO2 enriched area.•CO2 sequestering efficiency was confirmed by CA and RuBisCO enzyme assay.•Bacterium produces enhanced biosurfactant by utilizing CO2 sequestration efficiency.•FTIR and NMR analysis revealed the presence of lipopeptide type biosurfactant.•Biosurfactant production was optimized by Response Surface Methodology. Carbon dioxide utilizing bacterium Bacillus sp. SS105 was isolated from FACE (free air CO2 enriched) sample. The strain was grown in shake flask containing minimal salt medium with 50mM NaHCO3 as autotrophic carbon source and molasses as a low cost byproduct for mixotrophic growth. Carbon dioxide sequestration property of Bacillus sp. SS105 was determined by enzyme assay of carbonic anhydrase and ribulose-1, 5-bisphosphate carboxylase/oxygenase (RuBisCO). Along with CO2 sequestration this strain produced biosurfactant and its characterization by FTIR and 1H NMR indicated lipopeptide nature. Optimization of process parameter along with nutrient sources for higher biosurfactant production was done by Response Surface Methodology (RSM). Under optimized conditions, the yield of biosurfactant and biomass was 2.65 and 2.78gL−1 respectively. The study revealed simultaneous CO2 sequestration and biosurfactant production by Bacillus sp. SS105.