Angiopoietin-like protein 3 and 4 expression 4 and their serum levels in hepatocellular carcinoma

• Published in: Cytokine (Philadelphia, Pa.) Pa.), 2017-08, Vol.96, p.75-86
• Main Authors: El-Shal, Amal S., Zidan, Haidy E., Rashad, Nearmeen M., Wadea, Fady M.
• Format: Article
• Language: English
• Subjects: Aged; Angiopoietin-like 4 Protein - blood; Angiopoietin-like 4 Protein - genetics; Angiopoietin-like protein-3; Angiopoietin-like protein-4; Angiopoietin-like Proteins - blood; Angiopoietin-like Proteins - genetics; Biomarkers, Tumor - blood; Carcinoma, Hepatocellular - blood; Case-Control Studies; Chronic hepatitis; Cirrhosis; Enzyme-Linked Immunosorbent Assay; Female; Hepatitis, Chronic - blood; Hepatitis, Chronic - diagnosis; Hepatocellular carcinoma; Humans; Liver Cirrhosis - blood; Liver Neoplasms - blood; Liver Neoplasms - diagnosis; Male; Middle Aged; Real-Time Polymerase Chain Reaction; ROC Curve
• ISSN: 1043-4666; 1096-0023
• DOI: 10.1016/j.cyto.2017.03.006

•ANGPTL3 expression was elevated in HCC compared to chronic hepatitis.•ANGPTL 4 expression was elevated in HCC compared to chronic hepatitis.•Higher serum ANGPTL3 and ANGPTL4 values in HCC and chronic hepatitis.•Increased ANGPTL3 expression, AFP, serum ANGPTL 3 were assosciatedassociated with HCC risk.•ANGPTL 3 expression and AFP levels were the only pridectorspredictors of HCC. Hepatocellular carcinoma (HCC) is the 6th most common cancer and the 3rd leading cause of cancer causing death allover the world. The aim of this research to explore the clinical relevance of blood angiopoietin-like protein-3 (ANGPTL3) and ANGPTL4 expression and their proteins levels as non invasive biomarkers in cirrhotic and HCC patients and their influence on the clinicopathological features of HCC. This work comprised 200 patients with chronic hepatitis (120 cases complicated with cirrhosis, 80 patients with primary HCC) and 100 controls. circulating ANGPTL3 and ANGPTL4 expression was estimated by real-time polymerase chain reaction (RT-PCR). ANGPTL3 and ANGPTL4 protein levels were determined by enzyme-linked immunosorbent assay (ELISA). The circulating ANGPTL3 and ANGPTL 4 expression was significantly elevated in HCC cases compared to chronic hepatitis patients and controls. There were much more serum ANGPTL3 and ANGPTL4 values in HCC and chronic hepatitis patients as compared to controls, but we couldn't detect this significance between chronic hepatitis and HCC cases as regards ANGPTL4. By Multiple stepwise linear regression analysis, an increased ANGPTL3 expression, alpha-fetoprotein (AFP), serum ANGPTL 3 levels, Child–Pugh grade were significantly assosciatedassociated with increased risk of HCC. Logistic regression analysis revealed that ANGPTL 3 expression and AFP levels were the only pridectorspredictors of HCC (odd's ratio (OR)=8.9; 8.6 respectively, P=0.003). Receiver operator characteristic (ROC) demonsterated that serum ANGPTL3 and ANGPTL4 levels were usufuluseful biomarkers discriminating chronic hepatitis cases from controls (AUC=0.820,0.887, respectively P