The effects of ozone and probiotics on the survival of black tiger shrimp ( Penaeus monodon)

• Published in: Aquaculture 2003-04, Vol.220 (1), p.437-448
• Main Authors: Meunpol, Oraporn, Lopinyosiri, Kanyajit, Menasveta, Piamsak
• Format: Article
• Language: English
• Subjects: Animal aquaculture; Animal productions; Aquaculture; Bacillus; Biological and medical sciences; Crustacea; Fundamental and applied biological sciences. Psychology; Invertebrate aquaculture; Marine; Ozone; Penaeus monodon; Probiotics; Shellfish; Vibrio harveyi
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/S0044-8486(02)00586-0

Total ozone production (TOP) from an ozonator, residual ozone concentration (ROC) in water, and the effects of ozone with or without probiotic supplemented feeds on bacterial growth, and shrimp ( Penaeus monodon) survival were investigated. Minimal effective ROC to inhibit 3 log units of Vibrio harveyi D331 for 6 h and 2 log units of Bacillus S11 for 9 h was 0.38 g O 3/l of ROC from 5-min ozonation. Shrimp postlarvae exposed to 0.34–0.50 mg O 3/l ROC (8-h ozonation) caused loss of balance, immobility and destruction of gill lamellar epithelium. In vivo treatment of juvenile P. monodon reared on probiotic feed for 1 month revealed that 0.35 mg O 3/l ROC (30-min ozonation) effectively inhibited 3 log units of V. harveyi D331 for 24 h. At this ROC dosage, there was no effect either on shrimp or on intestinal probiotics ( Bacillus S11). Shrimp survival from probiotic treatment, coupling with ozonation, increased significantly ( P≤0.05) compared with controls.