A cost-effective method of preparing larval fish otoliths for reading using enzyme digestion and staining

A fast and cost-effective method for examining otoliths in fish larvae was developed whereby the otolith remains in situ. Whole fish of the clownfish Amphiprion melanopus were enzyme-cleared using a laundry pre-soak and then stained using the Von Kossa silver staining method for calcium. The otolith...

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Published in:Journal of fish biology 2002-12, Vol.61 (6), p.1600-1605
Main Authors: GREEN, B. S, REILLY, S. M, MCCORMICK, M. I
Format: Article
Language:English
Subjects:
Amphiprion melanopus
Animal, plant and microbial ecology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
General aspects. Techniques
Marine
Methods and techniques (sampling, tagging, trapping, modelling...)
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creator GREEN, B. S
REILLY, S. M
MCCORMICK, M. I
description A fast and cost-effective method for examining otoliths in fish larvae was developed whereby the otolith remains in situ. Whole fish of the clownfish Amphiprion melanopus were enzyme-cleared using a laundry pre-soak and then stained using the Von Kossa silver staining method for calcium. The otolith nucleus, daily rings and the otolith edge were all clearly visible and were suitable for a variety of age and growth analyses. The total hands on' time required to process these otoliths was c. 3min, and multiple samples could be processed simultaneously. The reduction in labour of this method to produce clear daily rings in the otolith lends itself to broad use in fish biology where large quantities of otoliths need to be examined in a cost- and time-efficient manner.Copyright 2002 The Fisheries Society of the British Isles. Published by Elsevier Science Ltd. All rights reserved.
doi_str_mv 10.1006/jfbi.2002.2177
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The reduction in labour of this method to produce clear daily rings in the otolith lends itself to broad use in fish biology where large quantities of otoliths need to be examined in a cost- and time-efficient manner.Copyright 2002 The Fisheries Society of the British Isles. Published by Elsevier Science Ltd. All rights reserved.</description><subject>Amphiprion melanopus</subject><subject>Animal, plant and microbial ecology</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects. 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source Wiley-Blackwell Read & Publish Collection
subjects Amphiprion melanopus
Animal, plant and microbial ecology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
General aspects. Techniques
Marine
Methods and techniques (sampling, tagging, trapping, modelling...)
title A cost-effective method of preparing larval fish otoliths for reading using enzyme digestion and staining
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