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Protective effect of glutamine on the main and adjacent organs damaged by ischemia-reperfusion in rats
Intestinal ischemia and reperfusion (I/R) causes cellular and tissue damage to the intestine and remote organs such as the liver. Increased production of ROS and nitric oxide and dysregulation of cytoprotective enzymes may be involved in intestinal I/R. The aim was to evaluate the protective effects...
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| Published in: | Protoplasma 2017-11, Vol.254 (6), p.2155-2168 |
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| creator | Hartmann, Renata Minuzzo Licks, Francielli Schemitt, Elizângela Gonçalves Colares, Josieli Raskopf do Couto Soares, Mariana Zabot, Gilmara Pandolfo Fillmann, Henrique Sarubbi Marroni, Norma Possa |
| description | Intestinal ischemia and reperfusion (I/R) causes cellular and tissue damage to the intestine and remote organs such as the liver. Increased production of ROS and nitric oxide and dysregulation of cytoprotective enzymes may be involved in intestinal I/R. The aim was to evaluate the protective effects of glutamine on the intestine and liver of rats with intestinal I/R injury. Twenty male Wistar rats (300 g) were divided into four groups: sham-operated (SO), glutamine + SO (G + SO), I/R, and glutamine + I/R (G + I/R). Occlusion of the SMA for 30 min was followed by 15-min reperfusion. Glutamine (25 mg/kg/day) was administered once daily 24 and 48 h before I/R induction. Blood and tissue of were collected for aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels, histopathological analysis, immunohistochemistry of IL-1β and TNF-α, thiobarbituric acid reactive substance (TBARS) and nitric oxide, Nrf2/keap1, superoxide dismutase (SOD), NADPH quinone oxidoreductase1 (NQO1), inducible nitric oxide synthase (iNOS), heat shock protein (HSP70), glucose-regulated protein 78 (GRP78), and activating transcription factor 6 (ATF-6) by western blot. Statistic analysis by ANOVA–Student-Newman-Keuls test (mean ± SE) significantly was
p
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| doi_str_mv | 10.1007/s00709-017-1102-3 |
| format | article |
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p
< 0.05. Tissue damage, AST, ALT, IL-1β, TNF-α, TBARS, NO, Keap1, iNOS, GRP78, and ATF-6 expression were significantly lower in the G + I/R group as compared to the I/R group. Expression of Nrf2, SOD, NQO1, and HSP70, was significantly higher in the G + I/R group as compared to I/R group. Pre-treatment with glutamine provided protection against oxidative damage in the intestine and liver in an experimental model of intestinal I/R.</description><identifier>ISSN: 0033-183X</identifier><identifier>EISSN: 1615-6102</identifier><identifier>DOI: 10.1007/s00709-017-1102-3</identifier><identifier>PMID: 28382390</identifier><language>eng</language><publisher>Vienna: Springer Vienna</publisher><subject>Activating transcription factor 6 ; Activating Transcription Factor 6 - metabolism ; Alanine ; Alanine transaminase ; Alanine Transaminase - blood ; Animals ; Aspartate aminotransferase ; Aspartate Aminotransferases - blood ; Biomedical and Life Sciences ; Cell Biology ; Drug Evaluation, Preclinical ; Glutamine ; Glutamine - pharmacology ; Glutamine - therapeutic use ; Heat shock proteins ; Hsp70 protein ; IL-1β ; Immunohistochemistry ; Intestine ; Intestines - blood supply ; Intestines - drug effects ; Intestines - pathology ; Ischemia ; Life Sciences ; Lipid Peroxidation ; Liver ; Liver - drug effects ; Liver - pathology ; Male ; NADP ; Nitrates - metabolism ; Nitric oxide ; Nitric Oxide Synthase Type II - metabolism ; Nitric-oxide synthase ; Nitrites - metabolism ; Occlusion ; Original Article ; Oxidative Stress ; Plant Sciences ; Protective Agents - pharmacology ; Protective Agents - therapeutic use ; Quinones ; Rats, Wistar ; Reactive oxygen species ; Reperfusion ; Reperfusion Injury - blood ; Reperfusion Injury - prevention & control ; Rodents ; Superoxide dismutase ; Thiobarbituric acid ; Tumor necrosis factor-α ; Zoology</subject><ispartof>Protoplasma, 2017-11, Vol.254 (6), p.2155-2168</ispartof><rights>Springer-Verlag Wien 2017</rights><rights>Protoplasma is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-fd348b233152a0f8d5b9f8adfda0e1af4eac87db7b83d62bd47742f9f55f20863</citedby><cites>FETCH-LOGICAL-c372t-fd348b233152a0f8d5b9f8adfda0e1af4eac87db7b83d62bd47742f9f55f20863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28382390$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hartmann, Renata Minuzzo</creatorcontrib><creatorcontrib>Licks, Francielli</creatorcontrib><creatorcontrib>Schemitt, Elizângela Gonçalves</creatorcontrib><creatorcontrib>Colares, Josieli Raskopf</creatorcontrib><creatorcontrib>do Couto Soares, Mariana</creatorcontrib><creatorcontrib>Zabot, Gilmara Pandolfo</creatorcontrib><creatorcontrib>Fillmann, Henrique Sarubbi</creatorcontrib><creatorcontrib>Marroni, Norma Possa</creatorcontrib><title>Protective effect of glutamine on the main and adjacent organs damaged by ischemia-reperfusion in rats</title><title>Protoplasma</title><addtitle>Protoplasma</addtitle><addtitle>Protoplasma</addtitle><description>Intestinal ischemia and reperfusion (I/R) causes cellular and tissue damage to the intestine and remote organs such as the liver. Increased production of ROS and nitric oxide and dysregulation of cytoprotective enzymes may be involved in intestinal I/R. The aim was to evaluate the protective effects of glutamine on the intestine and liver of rats with intestinal I/R injury. Twenty male Wistar rats (300 g) were divided into four groups: sham-operated (SO), glutamine + SO (G + SO), I/R, and glutamine + I/R (G + I/R). Occlusion of the SMA for 30 min was followed by 15-min reperfusion. Glutamine (25 mg/kg/day) was administered once daily 24 and 48 h before I/R induction. Blood and tissue of were collected for aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels, histopathological analysis, immunohistochemistry of IL-1β and TNF-α, thiobarbituric acid reactive substance (TBARS) and nitric oxide, Nrf2/keap1, superoxide dismutase (SOD), NADPH quinone oxidoreductase1 (NQO1), inducible nitric oxide synthase (iNOS), heat shock protein (HSP70), glucose-regulated protein 78 (GRP78), and activating transcription factor 6 (ATF-6) by western blot. Statistic analysis by ANOVA–Student-Newman-Keuls test (mean ± SE) significantly was
p
< 0.05. Tissue damage, AST, ALT, IL-1β, TNF-α, TBARS, NO, Keap1, iNOS, GRP78, and ATF-6 expression were significantly lower in the G + I/R group as compared to the I/R group. Expression of Nrf2, SOD, NQO1, and HSP70, was significantly higher in the G + I/R group as compared to I/R group. Pre-treatment with glutamine provided protection against oxidative damage in the intestine and liver in an experimental model of intestinal I/R.</description><subject>Activating transcription factor 6</subject><subject>Activating Transcription Factor 6 - metabolism</subject><subject>Alanine</subject><subject>Alanine transaminase</subject><subject>Alanine Transaminase - blood</subject><subject>Animals</subject><subject>Aspartate aminotransferase</subject><subject>Aspartate Aminotransferases - blood</subject><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Drug Evaluation, Preclinical</subject><subject>Glutamine</subject><subject>Glutamine - pharmacology</subject><subject>Glutamine - therapeutic use</subject><subject>Heat shock proteins</subject><subject>Hsp70 protein</subject><subject>IL-1β</subject><subject>Immunohistochemistry</subject><subject>Intestine</subject><subject>Intestines - blood supply</subject><subject>Intestines - drug effects</subject><subject>Intestines - pathology</subject><subject>Ischemia</subject><subject>Life Sciences</subject><subject>Lipid Peroxidation</subject><subject>Liver</subject><subject>Liver - drug effects</subject><subject>Liver - pathology</subject><subject>Male</subject><subject>NADP</subject><subject>Nitrates - metabolism</subject><subject>Nitric oxide</subject><subject>Nitric Oxide Synthase Type II - metabolism</subject><subject>Nitric-oxide synthase</subject><subject>Nitrites - metabolism</subject><subject>Occlusion</subject><subject>Original Article</subject><subject>Oxidative Stress</subject><subject>Plant Sciences</subject><subject>Protective Agents - pharmacology</subject><subject>Protective Agents - therapeutic use</subject><subject>Quinones</subject><subject>Rats, Wistar</subject><subject>Reactive oxygen species</subject><subject>Reperfusion</subject><subject>Reperfusion Injury - blood</subject><subject>Reperfusion Injury - prevention & control</subject><subject>Rodents</subject><subject>Superoxide dismutase</subject><subject>Thiobarbituric acid</subject><subject>Tumor necrosis factor-α</subject><subject>Zoology</subject><issn>0033-183X</issn><issn>1615-6102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp1kcFLHTEQxkOp1KftH9CLBHrxkjpJNrvZY5FWBaEeFHoLs5vJcx9vs89kt-B_b-SpSKGXyYT8vm-GfIx9lfBdAjRnuRRoBchGSAlK6A9sJWtpRF1uH9kKQGshrf5zyI5y3gCAUWA-sUNltVW6hRULN2maqZ-Hv8QphNLxKfD1dplxHCLxKfL5nviIQ-QYPUe_wZ5iodIaY-YeR1yT590jH3J_T-OAItGOUljyUMRFlnDOn9lBwG2mLy_nMbv79fP2_FJc_764Ov9xLXrdqFkEryvbKa2lUQjBetO1waIPHoEkhoqwt43vms5qX6vOV01TqdAGY4ICW-tjdrr33aXpYaE8u7GsRdstRpqW7KS1lbWtbUxBv_2DbqYlxbKdk62pjKmrShZK7qk-TTknCm6XhhHTo5PgnkNw-xBcCcE9h-B00Zy8OC_dSP5N8frrBVB7IJenuKb0bvR_XZ8AttKSbw</recordid><startdate>20171101</startdate><enddate>20171101</enddate><creator>Hartmann, Renata Minuzzo</creator><creator>Licks, Francielli</creator><creator>Schemitt, Elizângela Gonçalves</creator><creator>Colares, Josieli Raskopf</creator><creator>do Couto Soares, Mariana</creator><creator>Zabot, Gilmara Pandolfo</creator><creator>Fillmann, Henrique Sarubbi</creator><creator>Marroni, Norma Possa</creator><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20171101</creationdate><title>Protective effect of glutamine on the main and adjacent organs damaged by ischemia-reperfusion in rats</title><author>Hartmann, Renata Minuzzo ; Licks, Francielli ; Schemitt, Elizângela Gonçalves ; Colares, Josieli Raskopf ; do Couto Soares, Mariana ; Zabot, Gilmara Pandolfo ; Fillmann, Henrique Sarubbi ; Marroni, Norma Possa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-fd348b233152a0f8d5b9f8adfda0e1af4eac87db7b83d62bd47742f9f55f20863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Activating transcription factor 6</topic><topic>Activating Transcription Factor 6 - metabolism</topic><topic>Alanine</topic><topic>Alanine transaminase</topic><topic>Alanine Transaminase - blood</topic><topic>Animals</topic><topic>Aspartate aminotransferase</topic><topic>Aspartate Aminotransferases - blood</topic><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Drug Evaluation, Preclinical</topic><topic>Glutamine</topic><topic>Glutamine - pharmacology</topic><topic>Glutamine - therapeutic use</topic><topic>Heat shock proteins</topic><topic>Hsp70 protein</topic><topic>IL-1β</topic><topic>Immunohistochemistry</topic><topic>Intestine</topic><topic>Intestines - blood supply</topic><topic>Intestines - drug effects</topic><topic>Intestines - pathology</topic><topic>Ischemia</topic><topic>Life Sciences</topic><topic>Lipid Peroxidation</topic><topic>Liver</topic><topic>Liver - drug effects</topic><topic>Liver - pathology</topic><topic>Male</topic><topic>NADP</topic><topic>Nitrates - metabolism</topic><topic>Nitric oxide</topic><topic>Nitric Oxide Synthase Type II - 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Academic</collection><jtitle>Protoplasma</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hartmann, Renata Minuzzo</au><au>Licks, Francielli</au><au>Schemitt, Elizângela Gonçalves</au><au>Colares, Josieli Raskopf</au><au>do Couto Soares, Mariana</au><au>Zabot, Gilmara Pandolfo</au><au>Fillmann, Henrique Sarubbi</au><au>Marroni, Norma Possa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protective effect of glutamine on the main and adjacent organs damaged by ischemia-reperfusion in rats</atitle><jtitle>Protoplasma</jtitle><stitle>Protoplasma</stitle><addtitle>Protoplasma</addtitle><date>2017-11-01</date><risdate>2017</risdate><volume>254</volume><issue>6</issue><spage>2155</spage><epage>2168</epage><pages>2155-2168</pages><issn>0033-183X</issn><eissn>1615-6102</eissn><abstract>Intestinal ischemia and reperfusion (I/R) causes cellular and tissue damage to the intestine and remote organs such as the liver. Increased production of ROS and nitric oxide and dysregulation of cytoprotective enzymes may be involved in intestinal I/R. The aim was to evaluate the protective effects of glutamine on the intestine and liver of rats with intestinal I/R injury. Twenty male Wistar rats (300 g) were divided into four groups: sham-operated (SO), glutamine + SO (G + SO), I/R, and glutamine + I/R (G + I/R). Occlusion of the SMA for 30 min was followed by 15-min reperfusion. Glutamine (25 mg/kg/day) was administered once daily 24 and 48 h before I/R induction. Blood and tissue of were collected for aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels, histopathological analysis, immunohistochemistry of IL-1β and TNF-α, thiobarbituric acid reactive substance (TBARS) and nitric oxide, Nrf2/keap1, superoxide dismutase (SOD), NADPH quinone oxidoreductase1 (NQO1), inducible nitric oxide synthase (iNOS), heat shock protein (HSP70), glucose-regulated protein 78 (GRP78), and activating transcription factor 6 (ATF-6) by western blot. Statistic analysis by ANOVA–Student-Newman-Keuls test (mean ± SE) significantly was
p
< 0.05. Tissue damage, AST, ALT, IL-1β, TNF-α, TBARS, NO, Keap1, iNOS, GRP78, and ATF-6 expression were significantly lower in the G + I/R group as compared to the I/R group. Expression of Nrf2, SOD, NQO1, and HSP70, was significantly higher in the G + I/R group as compared to I/R group. Pre-treatment with glutamine provided protection against oxidative damage in the intestine and liver in an experimental model of intestinal I/R.</abstract><cop>Vienna</cop><pub>Springer Vienna</pub><pmid>28382390</pmid><doi>10.1007/s00709-017-1102-3</doi><tpages>14</tpages></addata></record> |
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| subjects | Activating transcription factor 6 Activating Transcription Factor 6 - metabolism Alanine Alanine transaminase Alanine Transaminase - blood Animals Aspartate aminotransferase Aspartate Aminotransferases - blood Biomedical and Life Sciences Cell Biology Drug Evaluation, Preclinical Glutamine Glutamine - pharmacology Glutamine - therapeutic use Heat shock proteins Hsp70 protein IL-1β Immunohistochemistry Intestine Intestines - blood supply Intestines - drug effects Intestines - pathology Ischemia Life Sciences Lipid Peroxidation Liver Liver - drug effects Liver - pathology Male NADP Nitrates - metabolism Nitric oxide Nitric Oxide Synthase Type II - metabolism Nitric-oxide synthase Nitrites - metabolism Occlusion Original Article Oxidative Stress Plant Sciences Protective Agents - pharmacology Protective Agents - therapeutic use Quinones Rats, Wistar Reactive oxygen species Reperfusion Reperfusion Injury - blood Reperfusion Injury - prevention & control Rodents Superoxide dismutase Thiobarbituric acid Tumor necrosis factor-α Zoology |
| title | Protective effect of glutamine on the main and adjacent organs damaged by ischemia-reperfusion in rats |
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