Different effects of neuropeptide Y on proliferation of vascular smooth muscle cells via regulation of Geminin

• Published in: Molecular and cellular biochemistry 2017-09, Vol.433 (1-2), p.205-211
• Main Authors: Jiang, Zhou-qin, Zhou, You-li, Chen, Xia, Li, Lin-yu, Liang, Shi-yu, Lin, Shu, Shu, Mao-qin
• Format: Article
• Language: English
• Subjects: Analysis; Arteriosclerosis; Atherosclerosis; Biochemistry; Biomedical and Life Sciences; Cardiology; Cell proliferation; Cells; Circulatory system; Cytometry; Development and progression; Down-regulation; Flow cytometry; Geminin; Life Sciences; Medical Biochemistry; Neuropeptide Y; Neuropeptides; Oncology; Pathogenesis; Polymerase chain reaction; Smooth muscle; Stimulation; Thymidine
• ISSN: 0300-8177; 1573-4919
• DOI: 10.1007/s11010-017-3028-7

The proliferation-promoting effect of neuropeptide Y (NPY) always functions in low-serum-cultured vascular smooth muscle cells (VSMCs), and the phenotypic switch of VSMCs is regulated by concentrations of serum. Whether the property of the NPY proliferative effect in VSMCs relies on phenotype of VSMCs is unclear. We aimed to explore the role of NPY on proliferation of different VSMC phenotypes in the pathogenesis of atherosclerosis. By stimulating A10 cells with 200 nM NPY in 0.5 or 10% serum, 3H-thymidine and 5-ethynyl-2′-deoxyuridine (EdU) and CCK8 measurements were used to detect VSMC proliferation. RT-PCR and Flow cytometry were performed to detect the factors involved in different properties of the NPY proliferative effect in VSMCs. Instead of facilitating proliferation, NPY had no significant effect on the growth of VSMCs when cultured in 10% serum (VSMCs stayed at synthetic states). The underlying mechanism may be involved in down-regulation of Y1 receptor ( P