Effects of sample storage and diluents in the reliability of thyroglobulin measurement in the washout of fine needle aspirates

Objective To evaluate two variables affecting Thyroglobulin stability in the washout of fine needle aspiration biopsies of thyroid nodules and metastatic lymph nodes. Materials and methods Thyroglobulin stability after storage at −20 °C up to 14 days was studied in washout performed with normal sali...

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Bibliographic Details
Published in:Endocrine 2017-06, Vol.56 (3), p.504-508
Main Authors: Piotrkowski-Viale, Fanny, Reyes, Adriana, Dios, Alicia, Pitoia, Fabian, Lowenstein, Alicia, Glikman, Patricia
Format: Article
Language:English
Subjects:
Antibodies
Benign
Biopsy, Fine-Needle
Bovine serum albumin
Calcitonin
Diabetes
Diluents
Endocrine Methods and Techniques
Endocrinology
Humanities and Social Sciences
Humans
Internal Medicine
Lymph nodes
Lymphatic system
Medicine
Medicine & Public Health
Metastases
Metastasis
multidisciplinary
Nodules
Parathyroid
Parathyroid hormone
Reproducibility of Results
Science
Sensitivity and Specificity
Shelf life
Specimen Handling - methods
Thyroglobulin
Thyroglobulin - analysis
Thyroid
Thyroid Function Tests
Thyroid gland
Thyroid Neoplasms - diagnosis
Thyroid Neoplasms - pathology
Thyroid Nodule - diagnosis
Thyroid Nodule - pathology
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Summary:Objective To evaluate two variables affecting Thyroglobulin stability in the washout of fine needle aspiration biopsies of thyroid nodules and metastatic lymph nodes. Materials and methods Thyroglobulin stability after storage at −20 °C up to 14 days was studied in washout performed with normal saline solution and further dilutions with normal saline solution from five metastatic thyroid nodes and six benign thyroid nodules. We also studied thyroglobulin stability in diluents compared with normal saline solution: 4% bovine serum albumin in normal saline solution and diluents free from thyroglobulin or Calcitonin or parathyroid hormone in paired and simultaneous thyroglobulin measurements of washout dilutions from 5/6 benign thyroid nodules and 2/5 metastatic lymph nodes. Thyroglobulin and Thyroglobulin antibodies were measured by a chemiluminescent assay. Positive samples with thyroglobulin antibodies in serum and/or washout were excluded. Results Thyroglobulin decreased with storage in washout or dilutions of washout performed in normal saline solution ( p  
ISSN:1355-008X
1559-0100
DOI:10.1007/s12020-017-1294-5