USP19 suppresses cellular type I interferon signaling by targeting TRAF3 for deubiquitination

To investigate host factors that mediate the immune escape of enterovirus 71 (EV71) in the context of deubiquitinating enzymes. Utilize PCR array to screen candidate genes that may be involved in EV71-induced cellular antiviral immune responses, and utilize protein mass spectrometry analysis to iden...

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Bibliographic Details
Published in:Future microbiology 2017-07, Vol.12 (9), p.767-779
Main Authors: Gu, Zhiwen, Shi, Weifeng, Zhang, Li, Hu, Zhilin, Xu, Chao
Format: Article
Language:English
Subjects:
deubiquitinating enzyme
Deubiquitinating Enzymes - metabolism
Endopeptidases - genetics
Endopeptidases - metabolism
enterovirus 71
Enterovirus A, Human - immunology
Gene expression
Genomes
Glycerol
HEK293 Cells
Humans
Immune Evasion
Immune response
Immunity, Cellular
Immunity, Innate
Immunoglobulins
Infections
Interferon
Interferon Type I - antagonists & inhibitors
Interferon Type I - metabolism
Kinases
Mass spectrometry
Mass spectroscopy
Oligonucleotide Array Sequence Analysis
Pattern recognition
Phosphatase
Plasmids
Protein Processing, Post-Translational
Proteins
Signal Transduction
TNF Receptor-Associated Factor 3 - genetics
TNF Receptor-Associated Factor 3 - metabolism
TRAF3
Tumor necrosis factor-TNF
type I interferon signaling
Ubiquitin
Ubiquitin-specific proteinase
Ubiquitination
USP19
Viral infections
Viruses
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Summary:To investigate host factors that mediate the immune escape of enterovirus 71 (EV71) in the context of deubiquitinating enzymes. Utilize PCR array to screen candidate genes that may be involved in EV71-induced cellular antiviral immune responses, and utilize protein mass spectrometry analysis to identify the functional targets of the candidate regulator. EV71 infection induces the upregulation of ubiquitin-specific protease 19 ( ) gene expression, which negatively regulates cellular antiviral type I interferon signaling. Additionally, we identify that USP19 suppresses cellular type I interferon signaling by targeting tumor necrosis factor receptor-associated factor 3 (TRAF3) molecule and decreasing TRAF3 ubiquitination of K63-linkage. This work suggests that USP19 is a previously unrecognized regulator employed by EV71 to evade host antiviral defenses.
ISSN:1746-0913
1746-0921
DOI:10.2217/fmb-2017-0006