Poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) production from biodiesel by‐product and propionic acid by mutant strains of Pandoraea sp

Pandoraea sp. MA03 wild type strain was subjected to UV mutation to obtain mutants unable to grow on propionic acid (PA) but still able to produce poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) [P(3HB‐co‐3HV)] from glycerol and PA at high 3HV yields. In shake flask experiments, mutant prp25 was select...

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Bibliographic Details
Published in:Biotechnology progress 2017-07, Vol.33 (4), p.1077-1084
Main Authors: de Paula, Fabrício C., de Paula, Carolina B. C., Gomez, José Gregório C., Steinbüchel, Alexander, Contiero, Jonas
Format: Article
Language:English
Subjects:
Batch culture
biodiesel
Biodiesel fuels
Biofuels
biopolymer
Burkholderiaceae - genetics
Burkholderiaceae - metabolism
Byproducts
Diesel
Glycerol
Metabolism
Mutants
Mutation
Pandoraea sp
Polyesters - chemistry
Polyesters - metabolism
polyhydroxyalkanoates
Propionates - chemistry
Propionates - metabolism
Propionic acid
Ultraviolet Rays
Valeric acid
Yield
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Summary:Pandoraea sp. MA03 wild type strain was subjected to UV mutation to obtain mutants unable to grow on propionic acid (PA) but still able to produce poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) [P(3HB‐co‐3HV)] from glycerol and PA at high 3HV yields. In shake flask experiments, mutant prp25 was selected from 52 mutants affected in the propionate metabolism exhibiting a conversion rate of PA into 3HV units of 0.78 g g−1. The use of crude glycerol (CG) plus PA or valeric acid resulted in a copolymer with 3HV contents varying from 21.9 to 30 mol% and 22.2 to 36.7 mol%, respectively. Fed‐batch fermentations were performed using CG and PA and reached a 3HV yield of 1.16 g g−1, which is 86% of the maximum theoretical yield. Nitrogen limitation was a key parameter for polymer accumulation reaching up to 63.7% content and 18.1 mol% of 3HV. Henceforth, mutant prp25 is revealed as an additional alternative to minimize costs and support the P(3HB‐co‐3HV) production from biodiesel by‐products. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1077–1084, 2017
ISSN:8756-7938
1520-6033
DOI:10.1002/btpr.2481