Photobiosynthesis of stable and functional silver/silver chloride nanoparticles with hydrolytic activity using hyperthermophilic β-glucosidases with industrial potential

• Published in: International journal of biological macromolecules 2017-09, Vol.102, p.84-91
• Main Authors: Araújo, Juscemácia N., Tofanello, Aryane, da Silva, Viviam M., Sato, Juliana A.P., Squina, Fabio M., Nantes, Iseli L., Garcia, Wanius
• Format: Article
• Language: English
• Subjects: beta-Glucosidase - chemistry; beta-Glucosidase - metabolism; Gram-Negative Anaerobic Straight, Curved, and Helical Rods - metabolism; Gram-Negative Anaerobic Straight, Curved, and Helical Rods - radiation effects; Green synthesis; Hyperthermostable β-glucosidase; Industry; Light; Metal Nanoparticles - chemistry; Models, Molecular; Nanoparticles; Protein Conformation; Silver - chemistry; Silver Compounds - chemistry; Silver Compounds - metabolism; Temperature
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2017.04.001

The β-glucosidases are important enzymes employed in a large number of processes and industrial applications, including biofuel production from biomass. Therefore, in this study, we reported for the first time the photobiosynthesis of stable and functional silver/silver chloride nanoparticles (Ag/AgCl-NPs) using two hyperthermostable bacterial β-glucosidases with industrial potential. The syntheses were straightforward and rapid processes carried out by mixing β-glucosidase and silver nitrate (in buffer 10mM Tris–HCl, pH 8) under irradiation with light (over a wavelength range of 450–600nm), therefore, compatible with the green chemistry procedure. Synthesized Ag/AgCl-NPs were characterized using a series of physical techniques. Absorption spectroscopy showed a strong absorption band centered at 460nm due to surface plasmon resonance of the Ag-NPs. X-ray diffraction analysis revealed that the Ag/AgCl-NPs were purely crystalline in nature. Under electron microscopy, Ag/AgCl-NPs of variable diameter ranging from 10 to 100nm can be visualized. Furthermore, electron microscopy, zeta potential and Fourier transform infrared spectroscopy results confirmed the presence of β-glucosidases coating and stabilizing the Ag/AgCl-NPs. Finally, the results showed that the enzymatic activities were maintained in the β-glucosidases assisted Ag/AgCl-NPs. The information described here should provide a useful basis for future studies of β-glucosidases assisted Ag/AgCl-NPs, including biotechnological applications.