Multiplex Real-Time PCR Method To Identify Shiga Toxin Genes stx1 and stx2 and Escherichia coli O157:H7/H super(-) Serotype

A multiplex real-time PCR method to simultaneously detect the stx1 and stx2 genes of Shiga toxin-producing Escherichia coli and a unique conserved single-nucleotide polymorphism in the E. coli O157:H7/H super(-) uidA gene has been developed. There is more than 98.6% sensitivity and 100% specificity...

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Bibliographic Details
Published in:Applied and environmental microbiology 2003-10, Vol.69 (10), p.6327-6333
Main Authors: Jinneman, K C, Yoshitomi, K J, Weagant, S D
Format: Article
Language:English
Online Access:Get full text
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Summary:A multiplex real-time PCR method to simultaneously detect the stx1 and stx2 genes of Shiga toxin-producing Escherichia coli and a unique conserved single-nucleotide polymorphism in the E. coli O157:H7/H super(-) uidA gene has been developed. There is more than 98.6% sensitivity and 100% specificity for all three gene targets based on a panel of 138 isolates. The PCR efficiencies were => 1.89, and as few as 6 CFU/reaction could be detected.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.69.10.6327-6333.2003