Development and optimization of a standard method for extraction of microplastics in mussels by enzyme digestion of soft tissues

The authors compared procedures for digestion of mussel soft tissues and extraction of microplastics. Complete tissue digestion was achieved with 1M NaOH, 35% HNO3, and protease at 9.6 UHb/mL (unit hemoglobin per mL); but use of HNO3 caused unacceptable destruction of some microplastics. Recovery of...

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Bibliographic Details
Published in:Environmental toxicology and chemistry 2017-04, Vol.36 (4), p.947-951
Main Authors: Catarino, Ana I., Thompson, Richard, Sanderson, William, Henry, Theodore B.
Format: Article
Language:English
Subjects:
Animals
Bivalvia - chemistry
Caustic digestion
Emerging pollutants
Environmental Monitoring - methods
Environmental Monitoring - standards
Environmental toxicology
Enzymatic digestion
Fourier transform infrared (FT‐IR)
Hemoglobin
Microplastics
Models, Theoretical
Mollusks
Mussels
Nitric Acid - chemistry
Optimization
Peptide Hydrolases - chemistry
Plastics - analysis
Recovery
Sodium hydroxide
Sodium Hydroxide - chemistry
Soft tissues
Water Pollutants, Chemical - analysis
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Summary:The authors compared procedures for digestion of mussel soft tissues and extraction of microplastics. Complete tissue digestion was achieved with 1M NaOH, 35% HNO3, and protease at 9.6 UHb/mL (unit hemoglobin per mL); but use of HNO3 caused unacceptable destruction of some microplastics. Recovery of microplastics spiked into mussels was similar (93 ± 10%) for NaOH and enzyme digestions. The authors recommend use of industrial enzymes based on digestion efficiency, microplastic recovery, and avoidance of caustic chemicals. Environ Toxicol Chem 2017;36:947–951. © 2016 SETAC
ISSN:0730-7268
1552-8618
DOI:10.1002/etc.3608