Application of microbial alkaloid prodigiosin as a potent matrix for the MALDI mass spectrometry analysis of low-molecular-weight plant antioxidants

Microbial alkaloid prodigiosin is proposed as a new effective matrix for the analysis of low-molecular- weight plant antioxidants by MALDI mass spectrometry in the negative ion detection mode. It is demonstrated that the physicochemical characteristics of prodigiosin, such as ability to activate ion...

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Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2016-12, Vol.71 (13), p.1233-1241
Main Authors: Duzhak, A. B., Williams, T. D., Panfilova, Z. I., Tsentalovich, Yu. P., Duzhak, T. G.
Format: Article
Language:English
Subjects:
Absorptivity
Alkaloids
Analysis
Analytical Chemistry
Antioxidants
Antioxidants (Nutrients)
Chemical properties
Chemistry
Chemistry and Materials Science
Crystallization
Desorption
Health aspects
High resolution
Ionization
Ions
Low molecular weight heparin
Mass spectra
Mass spectrometry
Mass spectroscopy
Microorganisms
Plant metabolism
Reproducibility
Scientific imaging
Stability analysis
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Summary:Microbial alkaloid prodigiosin is proposed as a new effective matrix for the analysis of low-molecular- weight plant antioxidants by MALDI mass spectrometry in the negative ion detection mode. It is demonstrated that the physicochemical characteristics of prodigiosin, such as ability to activate ionization/ desorption of analyte, high absorption coefficient at the working wavelengths of lasers, good solubility in solvents used for analytes, uniformity of crystallization on the target, stability under working conditions, and the absence of interfering peaks of fragment and cluster ions, are consistent with the recommendations for effective matrices. Prodigiosin as a matrix ensures the high-resolution detection of various plant antioxidants in complex mixtures. The obtained mass spectra of flavonoid aglycones were characterized by high quality (signal-to-noise ratio higher than 10 3 ) and reproducibility. The use of prodigiosin for analyzing a complex preparation of plant antioxidants ensures the simultaneous recording and interpretation of more than two dozens of ions corresponding to various antioxidants with the molecular weights of 100–500 Da.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934816130049