Construction of AIEgens-Based Bioprobe with Two Fluorescent Signals for Enhanced Monitor of Extracellular and Intracellular Telomerase Activity

Detections of telomerase activity in vitro and in living cells are of great importance for clinical diagnosis of cancer. In this work, an AIEgens-based bioprobe with two fluorescent signals for enhanced monitor of extracellular and intracellular telomerase activity is designed. After addition of tel...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2017-02, Vol.89 (3), p.2073-2079
Main Authors: Zhuang, Yuan, Shang, Chunli, Lou, Xiaoding, Xia, Fan
Format: Article
Language:English
Subjects:
Binding sites
Biosensing Techniques
Cancer
Cell Line, Tumor
Cells (biology)
Diagnosis
False Positive Reactions
Fluorescence
Fluorescent Dyes - chemistry
Humans
In vitro testing
Limit of Detection
Medical diagnosis
Molecular Imaging - methods
Monitors
Oncology
Signal transduction
Silanes - chemistry
Substrate Specificity
Telomerase
Telomerase - metabolism
Urinary Bladder Neoplasms - enzymology
Urine
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Summary:Detections of telomerase activity in vitro and in living cells are of great importance for clinical diagnosis of cancer. In this work, an AIEgens-based bioprobe with two fluorescent signals for enhanced monitor of extracellular and intracellular telomerase activity is designed. After addition of telomerase, two positively charged AIEgens (Silole-R and TPE-H) bind to quencher group labeled primer (QP) and the extension repeated units, leading enhancement of two telomerase-triggered fluorescent signals. Furthermore, by combination the wider linear range in vitro and lower background in living cells imaging, the bioprobe is used to detect telomerase extracted from various cell lines (MCF-7, HeLa, E-J, and HLF), 50 bladder cancer patients’ urine samples, 10 normal people’s urine samples, and also applied in mapping telomerase activity inside living cells (MCF-7, HeLa, MDA-MB-231, and HT1080). The results show that this well-designed strategy can successfully detect telomerase activity in vitro and in living cells with high sensitivity, indicating the potential application of this method in cancer cells bioimaging and clinical cancer diagnosis.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.6b04696