Optimization of Prochlorothrix hollandica cyanobacteria culturing for obtaining myristoleic acid

Plankton filament cyanobacteria Prochlorothrix hollandica is characterized by a very high content of C 14 and C 16 fatty acids (FA) in the lipid membranes. Depending on culturing conditions of the cyanobacteria, total concentrations of myristic and myristoleic acids can reach 35% and those of palmit...

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Bibliographic Details
Published in:Russian journal of plant physiology 2016-07, Vol.63 (4), p.558-565
Main Authors: Lapina, S. S., Maslova, I. P., Pchelkin, V. P., Bedbenov, V. S., Mironov, K. S., Zayadan, B. K., Userbaeva, A. A., Sinetova, M. A., Los, D. A.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Cyanobacteria
Life Sciences
Plant Physiology
Plant Sciences
Prochlorothrix hollandica
Research Papers
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Summary:Plankton filament cyanobacteria Prochlorothrix hollandica is characterized by a very high content of C 14 and C 16 fatty acids (FA) in the lipid membranes. Depending on culturing conditions of the cyanobacteria, total concentrations of myristic and myristoleic acids can reach 35% and those of palmitic and palmitoleic acids can reach 60% of all esterified FA cells. In P. hollandica , a variety of monounsaturated FA is represented by myristoleic and palmitic acids, and by hexadecenoic (C 16:1 ) acid with olefin bond of cis -configuration, located in the Δ4 position. The process of intensive culturing for P. hollandica cells to yield a maximal biomass in order to isolate the pure drug of myristoleic acid derivative has been optimized. The use of a threestage purification gives 30 mg of chromatographically pure myristoleic acid methyl ester from 17 g of P. hollandica raw biomass (dry mass is 3 g), which is 1% of dry cell mass.
ISSN:1021-4437
1608-3407
DOI:10.1134/S1021443716040087