TLR4-mediated inflammation is a key pathogenic event leading to kidney damage and fibrosis in cyclosporine nephrotoxicity

• Published in: Archives of toxicology 2017-04, Vol.91 (4), p.1925-1939
• Main Authors: González-Guerrero, Cristian, Cannata-Ortiz, Pablo, Guerri, Consuelo, Egido, Jesús, Ortiz, Alberto, Ramos, Adrián M.
• Format: Article
• Language: English
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Cyclosporine - toxicity; Cytokines - metabolism; Environmental Health; Fibrosis; HMGB1 Protein - metabolism; Immunosuppressive Agents - toxicity; Inflammation; Inflammation - chemically induced; Inflammation - pathology; Kidney - cytology; Kidney - drug effects; Kidney - pathology; Kidneys; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Neurotoxicity; Occupational Medicine/Industrial Medicine; Organ Toxicity and Mechanisms; Pathogens; Pharmacology/Toxicology; Rats; Sulfonamides - pharmacology; Toll-Like Receptor 4 - genetics; Toll-Like Receptor 4 - metabolism
• ISSN: 0340-5761; 1432-0738
• DOI: 10.1007/s00204-016-1830-8

Cyclosporine A (CsA) successfully prevents allograft rejection, but nephrotoxicity is still a dose-limiting adverse effect. TLR4 activation promotes kidney damage but whether this innate immunity receptor mediates CsA nephrotoxicity is unknown. The in vivo role of TLR4 during CsA nephrotoxicity was studied in mice co-treated with CsA and the TLR4 inhibitor TAK242 and also in TLR4 −/− mice. CsA-induced renal TLR4 expression in wild-type mice. Pharmacological or genetic targeting of TLR4 reduced the activation of proinflammatory signaling, including JNK/c-jun, JAK2/STAT3, IRE1α and NF-κB and the expression of Fn14. Expression of proinflammatory factors and cytokines was also decreased, and kidney monocyte and lymphocyte influx was prevented. TLR4 inhibition also reduced tubular damage and drastically prevented the development of kidney fibrosis. In vivo and in vitro CsA promoted secretion of the TLR ligand HMGB1 by tubular cells upstream of TLR4 activation, and prevention of HMGB1 secretion significantly reduced CsA-induced synthesis of MCP-1, suggesting that HMGB1 may be one of the mediators of CsA-induced TLR4 activation. These results suggest that TLR4 is a potential pharmacological target in CsA nephrotoxicity.