LncRNA NONRATT021972 involved the pathophysiologic processes mediated by P2X sub(7) receptors in stellate ganglia after myocardial ischemic injury

• Published in: Purinergic signalling 2016-03, Vol.12 (1), p.127-137
• Main Authors: Zou, Lifang, Tu, Guihua, Xie, Wei, Wen, Shiyao, Xie, Qiuyu, Liu, Shuangmei, Li, Guilin, Gao, Yun, Xu, Hong, Wang, Shouyu, Xue, Yun, Wu, Bing, Lv, Qiulan, Ying, Mofeng, Zhang, Xi, Liang, Shangdong
• Format: Article
• Language: English
• ISSN: 1573-9538; 1573-9546
• DOI: 10.1007/s11302-015-9486-z

Adenosine triphosphate (ATP) acts on P2X receptors to initiate signal transmission. P2X sub(7) receptors play a role in the pathophysiological process of myocardial ischemic injury. Long noncoding RNAs (lncRNAs) participate in numerous biological functions independent of protein translation. LncRNAs are implicated in nervous system diseases. This study investigated the effects of NONRATT021972 small interference RNA (siRNA) on the pathophysiologic processes mediated by P2X sub(7) receptors in stellate ganglia (SG) after myocardial ischemic injury. Our results demonstrated that the expression of NONRATT021972 in SG was significantly higher in the myocardial ischemic (MI) group than in the control group. Treatment of MI rats with NONRATT021972 siRNA, the P2X sub(7) antagonist brilliant blue G (BBG), or P2X sub(7) siRNA improved the histology of injured ischemic cardiac tissues and decreased the elevated concentrations of serum myocardial enzymes, creatine kinase (CK), CK isoform MB (CK-MB), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) compared to the MI rats. NONRATT021972 siRNA, BBG, or P2X sub(7) siRNA treatment in MI rats decreased the expression levels of P2X sub(7) immunoreactivity, P2X sub(7) messenger RNA (mRNA), and P2X sub(7) protein, interleukin-6 (IL-6), tumor necrosis factor- alpha (TNF- alpha ), and phosphorylated p38 mitogen-activated protein kinase (p38 MAPK) in the SG compared to MI rats. NONRATT021972 siRNA treatment prevented the pathophysiologic processes mediated by P2X sub(7) receptors in the SG after myocardial ischemic injury.