Innovative methods to generate clean sugar stream from biomass feedstocks for efficient fermentation

Sorghum stalks were evaluated to generate clean sugars; enzymatic hydrolysis efficiency of acid pretreated biomass was significantly low (12.3 g sugar/g pretreated biomass) compared to alkali method (49.1 g sugar/g pretreated biomass). Inhibitory compounds derived from acid pretreatment, such as hyd...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2017-04, Vol.40 (4), p.633-641
Main Authors: Lee, Jung-Eun, Guragain, Yadhu N., Bastola, Krishna P., Vadlani, Praveen V.
Format: Article
Language:English
Subjects:
Acetic acid
Activated charcoal
Biomass
Biotechnology
Carbohydrates - chemistry
Charcoal
Chemistry
Chemistry and Materials Science
Detoxification
Environmental Engineering/Biotechnology
Enzyme kinetics
Fermentation
Food Science
Hydrolysis
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Plant Stems - chemistry
Research Paper
Sorghum
Sorghum - chemistry
Sugar
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Summary:Sorghum stalks were evaluated to generate clean sugars; enzymatic hydrolysis efficiency of acid pretreated biomass was significantly low (12.3 g sugar/g pretreated biomass) compared to alkali method (49.1 g sugar/g pretreated biomass). Inhibitory compounds derived from acid pretreatment, such as hydroxymethyl furfural (HMF) and furfural, were effectively removed using an appropriate detoxification method using 1% (w/v) activated charcoal. The resin mixture containing cationic exchangers (DOWEX 50WX8) and anionic exchangers (IRA 743) in 7:3 ratio at pH 2.7 completely removed HMF, acetic acid and formic acid with less than 2% (w/w) sugar loss, leading to efficient detoxification of inhibitory compounds.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-016-1727-1