Detection of local inflammation induced by repeated exposure to contact allergens by use of IVIS SpectrumCT analyses

• Published in: Contact dermatitis 2017-04, Vol.76 (4), p.210-217
• Main Authors: Nielsen, Morten M., Schmidt, Jonas D., Christensen, Jan P., Geisler, Carsten, Johansen, Jeanne D., Bonefeld, Charlotte M.
• Format: Article
• Language: English
• Subjects: Allergens - administration & dosage; Allergens - adverse effects; Allergies; Animals; Cytokines; Dermatitis, Allergic Contact - diagnosis; Ear, External - drug effects; Ear, External - pathology; Female; Immunology; Inflammation; Inflammation - chemically induced; Inflammation - diagnosis; Irritants - administration & dosage; Irritants - adverse effects; IVIS SpectrumCT; local inflammation; Luminescent Measurements - methods; Male; Mice; Prosense 750 FAST; systemic inflammation
• ISSN: 0105-1873; 1600-0536
• DOI: 10.1111/cod.12746

Summary Background Contact allergy is characterized by local skin inflammation that, in some cases, can result in systemic immune activation. Objectives To investigate whether IVIS SpectrumCT analyses can be used to detect the immune response induced by contact allergens. Methods Mice were repeatedly exposed to vehicle or allergens on the ears. The local and systemic responses were analysed at different times with the ProSense 750 FAST probe in IVIS SpectrumCT measurements. In addition, changes in ear thickness, cytokine profile in the skin and immunological phenotype in the draining lymph nodes and spleen were determined. Results Local inflammation was detected by ProSense 750 FAST and correlated with changes in ear thickness, cytokine profile and immunological phenotype following exposure to the strong contact allergen 2,4‐dinitrofluorobenzene. Analysis of the systemic response with ProSense 750 FAST did not show any difference between allergen‐exposed and control mice, although fluorescence‐activated cell sorting analysis of the spleen showed increased numbers of γδ T cells and CD11b+CD11c+MHCII+ cells in allergen‐treated mice. Conclusions IVIS SpectrumCT analyses with ProSense 750 FAST as the probe can be used to detect local immune responses induced by contact allergens.