Application of theoretical methods to increase succinate production in engineered strains

Computational methods have enabled the discovery of non-intuitive strategies to enhance the production of a variety of target molecules. In the case of succinate production, reviews covering the topic have not yet analyzed the impact and future potential that such methods may have. In this work, we...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2017-04, Vol.40 (4), p.479-497
Main Authors: Valderrama-Gomez, M. A., Kreitmayer, D., Wolf, S., Marin-Sanguino, A., Kremling, A.
Format: Article
Language:English
Subjects:
Bioengineering
Biosynthesis
Biotechnology
Chemicals
Chemistry
Chemistry and Materials Science
Computational Biology
Critical Review
Environmental Engineering/Biotechnology
Food Science
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Metabolic Engineering - methods
Models, Biological
Succinic Acid - metabolism
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Summary:Computational methods have enabled the discovery of non-intuitive strategies to enhance the production of a variety of target molecules. In the case of succinate production, reviews covering the topic have not yet analyzed the impact and future potential that such methods may have. In this work, we review the application of computational methods to the production of succinic acid. We found that while a total of 26 theoretical studies were published between 2002 and 2016, only 10 studies reported the successful experimental implementation of any kind of theoretical knowledge. None of the experimental studies reported an exact application of the computational predictions. However, the combination of computational analysis with complementary strategies, such as directed evolution and comparative genome analysis, serves as a proof of concept and demonstrates that successful metabolic engineering can be guided by rational computational methods.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-016-1729-z