Surface physical chemistry properties in coated bacterial cellulose membranes with calcium phosphate

• Published in: Materials Science & Engineering C 2017-06, Vol.75, p.1359-1365
• Main Authors: de Olyveira, Gabriel Molina, Basmaji, Pierre, Costa, Ligia Maria Manzine, dos Santos, Márcio Luiz, dos Santos Riccardi, Carla, Guastaldi, Fernando Pozzi Semeghini, Scarel-Caminaga, Raquel Mantuaneli, de Oliveira Capote, Ticiana Sidorenko, Pizoni, Elisabeth, Guastaldi, Antônio Carlos
• Format: Article
• Language: English
• Subjects: Animals; Bacteria; Bacterial cellulose; Biomedical materials; Biomimetics; Calcium; Calcium phosphates; Calcium Phosphates - chemistry; Calcium Phosphates - pharmacology; Cell culture; Cell Survival - drug effects; Cellulose; Cellulose - chemistry; Cellulose - pharmacology; Chemical precipitation; CHO Cells; Chondroitin sulfate; Coated membrane; Cricetinae; Cricetulus; Cytotoxicity; Deposition; Fermentation; Inoculation; Materials science; Materials Testing; Membranes; Membranes, Artificial; Phosphate; Phosphates; Physical chemistry; Polysaccharides, Bacterial - chemistry; Polysaccharides, Bacterial - pharmacology; Regeneration; Regenerative medicine; Sulfates; Surface charge; Surface Properties; Tissue engineering; Tissue regeneration; Wettability; Wound healing; Wounds; X ray photoelectron spectroscopy
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2017.03.025

Bacterial cellulose has become established as a new biomaterial, and it can be used for medical applications. In addition, it has called attention due to the increasing interest in tissue engineering materials for wound care. In this work, the bacterial cellulose fermentation process was modified by the addition of chondroitin sulfate to the culture medium before the inoculation of the bacteria. The biomimetic process with heterogeneous calcium phosphate precipitation of biological interest was studied for the guided regeneration purposes on bacterial cellulose. FTIR results showed the incorporation of the chondroitin sulfate in the bacterial cellulose, SEM images confirmed the deposition of the calcium phosphate on the bacterial cellulose surface, XPS analysis showed a selective chemical group influences which change calcium phosphate deposition, besides, the calcium phosphate phase with different Ca/P ratios on bacterial cellulose surface influences wettability. XTT results concluded that these materials did not affect significantly in the cell viability, being non-cytotoxic. Thus, it was produced one biomaterial with the surface charge changes for calcium phosphate deposition, besides different wettability which builds new membranes for Guided Tissue Regeneration. [Display omitted] •FTIR results showed incorporation of chondroitin sulfate in bacterial cellulose.•SEM images confirmed incorporation of calcium phosphate on BC surface.•XPS showed chemical group influences which change calcium phosphate deposition.•Wettability has influences of calcium phosphates with different Ca/P ratios.•XTT showed that materials did not affect cell viability, being non-cytotoxic.