Antibacterial activity of novel peptide derived from Cry1Ab16 toxin and development of LbL films for foodborne pathogens control

• Published in: Materials Science & Engineering C 2017-06, Vol.75, p.503-509
• Main Authors: Plácido, Alexandra, Bragança, Idalina, Marani, Mariela, de Araujo, Alyne Rodrigues, Vasconcelos, Andreanne Gomes, Batziou, Krystallenia, Domingues, Valentina F., Eaton, Peter, Almeida Leite, José Roberto S.A., Delerue-Matos, Cristina
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - chemistry; Antibacterial activity; Antimicrobial activity; Antimicrobial agents; Antimicrobial peptides; Atomic force microscopy; Bacillus thuringiensis - chemistry; Bacteria; Bacterial Proteins - chemistry; Biomaterials; Biomedical materials; Cry1Ab16 toxin; Developing countries; Diarrhea; E coli; Endotoxins - chemistry; Escherichia coli; Escherichia coli - growth & development; Etiology; Food safety; Foodborne Diseases - prevention & control; Foodborne pathogens; Hemolysin Proteins - chemistry; Infections; Intestine; LbL films; LDCs; Materials science; Membranes, Artificial; Microscopy; Pathogens; Peptides; Peptides - chemistry; Strains (organisms); Surgical implants
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2017.02.027

Escherichia coli is one of the most common etiological agents of diarrhea in developing countries. The appearance of resistant E. coli prevents treatment of these infections. Biotechnological products incorporating antimicrobial peptides are currently being considered in applications to prevent intestinal infections by these bacteria. The aim of this study was to evaluate the antibacterial activity of the peptide PcL342-354C, which is derived from the toxin Cry1Ab16 from Bacillus thuringiensis, against E. coli strains. We also report the preparation, characterization and evaluation of the antibacterial activity of LbL films containing PcL342-354C. The results showed that the PcL342-354C peptide inhibited the growth of different strains of E. coli with minimal inhibitory concentration ranging from 15.62–31.25μg/mL and minimal bactericidal concentration was 250μg/mL, indicating a potential antibacterial activity. The morphology of an ITO/Cashew gum/PcL342-354C film was analysed using atomic force microscopy which showed an increase of roughness due to the increase in the number of layers. The LbL films showed significant antibacterial activity against E. coli NCTC 9001 in both conditions tested (10 and 20 bilayers). Our results indicate that the peptide exhibits an antibacterial potential that can be tapped to develop biomaterials with antibacterial activity for use against foodborne pathogens. [Display omitted] •The PcL342–354C peptide inhibited the growth of E. coli.•The peptide can be simply incorporated into edible films combined with cashew gum.•LbL films incorporating the peptide have antibacterial activity against E. coli.•The PcL342–354C exhibits an antibacterial potential that can be tapped to develop biomaterials.