Pulmonary heat shock protein expression after exposure to a metabolically activated Clara cell toxicant: relationship to protein adduct formation

Heat shock proteins/stress proteins (Hsps) participate in regulation of protein synthesis and degradation and serve as general cytoprotectants, yet their role in lethal Clara cell injury is not clear. To define the pattern of Hsp expression in acute lethal Clara cell injury, mice were treated with t...

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Bibliographic Details
Published in:Toxicology and applied pharmacology 2003-10, Vol.192 (2), p.107-118
Main Authors: Williams, Kurt J, Cruikshank, Michael K, Plopper, Charles G
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blotting, Western
Bronchi - cytology
Bronchi - metabolism
Clara cell
Dose-Response Relationship, Drug
General aspects. Methods
Heat shock protein
Heat-Shock Proteins - biosynthesis
Heat-Shock Proteins - metabolism
Immunohistochemistry
Injections, Intraperitoneal
Male
Medical sciences
Mice
Naphthalene
Naphthalenes - toxicity
Protein adduct
Protein Binding
Time Factors
Toxicology
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Summary:Heat shock proteins/stress proteins (Hsps) participate in regulation of protein synthesis and degradation and serve as general cytoprotectants, yet their role in lethal Clara cell injury is not clear. To define the pattern of Hsp expression in acute lethal Clara cell injury, mice were treated with the Clara cell-specific toxicant naphthalene (NA), and patterns of expression compared to electrophilic protein adduction and previously established organellar degradation and gluathione (GSH) depletion. In sites of lethal injury (distal bronchiole), prior to organellar degradation (1 h post-NA), protein adduction is detectable and ubiquitin, Hsp 25, Hsp 72, and heme-oxygenase 1 (HO-1) are increased. Maximal Hsp expression, protein adduction, and GSH depletion occur simultaneous (by 2–3 h) with early organelle disruption. Hsp expression is higher later (6–24 h), only in exfoliating cells. In airway sites (proximal bronchiole) with nonlethal Clara cell injury elevation of Hsp 25, 72, and HO-1 expression follows significant GSH depletion (greater than 50% 2 h post-NA). This data build upon our previous studies and we conclude that (1) in lethal (terminal bronchiole) and nonlethal (proximal bronchiole) Clara cell injury, Hsp induction is associated with the loss of GSH and increased protein adduction, and (2) in these same sites, organelle disruption is not a prerequisite for Hsp induction.
ISSN:0041-008X
1096-0333
DOI:10.1016/S0041-008X(03)00302-8