Polygalacturonase produced by Botrytis fabae as elicitor of two furanoacetylenic phytoalexins in Vicia faba pods

Polygalacturonase, produced by Botrytis fabae in infected faba bean pods, was separated by isoelectric focusing and tested as possible elicitor of the phytoalexins wyerone acid and wyerone. The isoenzyme profile was characterised by multiple forms within a restricted range of isoelectric points (pI)...

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Bibliographic Details
Published in:Journal of plant pathology 2003-07, Vol.85 (2), p.111-116
Main Authors: Buzi, A, Chilosi, G, Magro, P. (Universita della Tuscia, Viterbo (Italy). Dipartimento di Protezione delle Piante), Timperio, A.M, Zolla, L. (Universita della Tuscia, Viterbo (Italy). Dipartimento di Scienze Ambientali), Rossall, S. (Nottingham Univ. (UK). School of Biosciences)
Format: Article
Language:English
Subjects:
Botrytis
BOTRYTIS FABAE
Endocarp
Enzymes
EXPERIMENTAL INFECTION
FITOALESSINE
FITOALEXINA
FOCALIZZAZIONE ISOELETTRICA
HOST PATHOGEN RELATIONS
INFECCIÓN EXPERIMENTAL
INFECTION EXPÉRIMENTALE
Infections
INFEZIONE SPERIMENTALE
ISOELECTRIC FOCUSING
ISOELECTROFOQUE
ISOENZIMAS
ISOENZIMI
ISOENZYME
ISOENZYMES
ISOÉLECTROFOCALISATION
Lesions
Liquids
Pathogens
PHYTOALEXINE
PHYTOALEXINS
Phytopathology
Plant cells
Plant tissues
POLIGALACTURONASA
POLIGALATTURONASI
POLYGALACTURONASE
RELACIONES HUESPED PATÓGENO
RELATION HÔTE PATHOGÈNE
RELAZIONI OSPITE PATOGENO
VICIA FABA
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Summary:Polygalacturonase, produced by Botrytis fabae in infected faba bean pods, was separated by isoelectric focusing and tested as possible elicitor of the phytoalexins wyerone acid and wyerone. The isoenzyme profile was characterised by multiple forms within a restricted range of isoelectric points (pI). These were indicated as very acidic, acidic and basic. Using liquid isoelectric focusing, polygalacturonase isoenzymes were separated into four different isoenzyme pools. These pools, tested at a range of concentrations, acted as an elicitor of wyerone acid and wyerone accumulation in endocarp pod tissues, the former being more prevalent. The level of phytoalexin accumulation varied depending on the enzymatic pool tested. Time course analysis revealed that phytoalexins accumulated at higher levels when lower enzyme doses were used. Each pool of isoenzymes was found to produce dark-brownish lesions similar to those observed from tissues inoculated with the pathogen. The possible dual role of polygalacturonase isoenzymes from B. fabae as putative pathogenicity determinants or defence responses elicitors during broad bean colonisation is discussed [La poligalatturonasi, prodotta da Botrytis fabae in legumi di fava infetti, e' stata separata mediante focalizzazione isoelettrica e saggiata come possibile stimolatore della produzione delle fitoalessine wyerone e acido wyeronico. Il profilo isoenzimatico era caratterizzato da forme multiple entro un intervallo ristretto di punti isoelettrici (pI). Questi erano indicati come molto acidi, acidi e basici. Utilizzando la focalizzazione isoelettrica liquida, gli isoenzimi della poligalatturonasi sono stati separati in quattro gruppi isoenzimatici. Questi gruppi, saggiati in un intervallo di concentrazioni, agivano come stimolatori dell'accumulo di acido wyeronico e wyerone nei tessuti dell'endocarpo dei legumi, con una prevalenza del primo. Il livello di accumulo di fitoalessine variava secondo il pool enzimatico sperimentato. L'analisi effettuata nel tempo ha evidenziato che le fitoalessine si accumulavano a livelli piu' elevati quando venivano utilizzate dosi inferiori di enzimi. Si e' riscontrato che ogni pool di isoenzimi produceva lesioni nero-brunastre simili a quelle osservate in tessuti inoculati con il patogeno. Viene discusso il possibile duplice ruolo degli isoenzimi della poligalatturonasi di B. fabae come determinanti putativi della patogenicita' o stimolatori di risposte di difesa nel corso della colonizzazi
ISSN:1125-4653
2239-7264