A fast fluorimetric assay (FFA) for the detection of saxitoxin in natural phytoplankton samples

A fast fluorimetric assay (FFA) for the detection of saxitoxins in plankton samples was compared with the analysis by high performance liquid chromatography (HPLC). The correlation between the results of the assay and those of the HPLC is significant for most of the carbamoyl saxitoxins. During rese...

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Bibliographic Details
Published in:Marine ecology. Progress series (Halstenbek) 2002-04, Vol.230, p.29-34
Main Authors: Gerdts, G., Hummert, C., Donner, G., Luckas, B., Schütt, C.
Format: Article
Language:English
Subjects:
Alexandrium
Algal blooms
Animal, plant and microbial ecology
Applied ecology
Biological and medical sciences
Cruises
Ecotoxicology, biological effects of pollution
Effects of pollution and side effects of pesticides on protozoa and invertebrates
Fluorescence
Fundamental and applied biological sciences. Psychology
General aspects. Techniques
Glass fibers
Marine
Methods and techniques (sampling, tagging, trapping, modelling...)
Nonesterified fatty acids
Saxitoxin
Shellfish
Toxicity
Toxins
Wavelengths
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Summary:A fast fluorimetric assay (FFA) for the detection of saxitoxins in plankton samples was compared with the analysis by high performance liquid chromatography (HPLC). The correlation between the results of the assay and those of the HPLC is significant for most of the carbamoyl saxitoxins. During research cruises to the Orkney Islands and the Firth of Forth (Scotland) in 1997 and 1998, samples of toxicAlexandriumspp. blooms were analyzed successfully by FFA and HPLC. Because the toxin pattern of toxicAlexandriumspp. mostly consists of highly fluorescent saxitoxins (like STX and GTX3), the assay can be applied for monitoring toxicAlexandriumspp. blooms and is suggested as a tool for future prewarning systems of paralytic shellfish poisoning (PSP) events.
ISSN:0171-8630
1616-1599
DOI:10.3354/meps230029