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CFTR-dependent defect in alternatively-activated macrophages in cystic fibrosis

Abstract Background The role of the macrophages in cystic fibrosis (CF) lung disease has been poorly studied. We hypothesized that alternatively activated M2 macrophages are abnormal in CF lung disease. Methods Blood samples were collected from adults (n = 13) children (n = 27) with CF on admission...

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Published in:Journal of cystic fibrosis 2017-07, Vol.16 (4), p.475-482
Main Authors: Tarique, Abdullah A, Sly, Peter D, Holt, Patrick G, Bosco, Anthony, Ware, Robert S, Logan, Jayden, Bell, Scott C, Wainwright, Claire E, Fantino, Emmanuelle
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cited_by cdi_FETCH-LOGICAL-c451t-87f9c2717fb164f67be51273e4c18284dafef26d6e202b8cd5827ce26b5098793
cites cdi_FETCH-LOGICAL-c451t-87f9c2717fb164f67be51273e4c18284dafef26d6e202b8cd5827ce26b5098793
container_end_page 482
container_issue 4
container_start_page 475
container_title Journal of cystic fibrosis
container_volume 16
creator Tarique, Abdullah A
Sly, Peter D
Holt, Patrick G
Bosco, Anthony
Ware, Robert S
Logan, Jayden
Bell, Scott C
Wainwright, Claire E
Fantino, Emmanuelle
description Abstract Background The role of the macrophages in cystic fibrosis (CF) lung disease has been poorly studied. We hypothesized that alternatively activated M2 macrophages are abnormal in CF lung disease. Methods Blood samples were collected from adults (n = 13) children (n = 27) with CF on admission for acute pulmonary exacerbation and when clinically stable. Monocytes were differentiated into macrophages and polarized into classical (M1) and alternatively-activated (M2) phenotypes, function determined ex-vivo and compared with healthy controls. Results In the absence of functional cystic fibrosis trans-membrane conductance regulator (CFTR), either naturally in patients with CF or induced with CFTR inhibitors, monocyte-derived macrophages do not respond to IL-13/IL-4, fail to polarize into M2s associated with a post-transcriptional failure to produce and express IL-13Rα1 on the macrophage surface Polarization to the M1 phenotype was unaffected. Conclusions CFTR-dependent imbalance of macrophage phenotypes and functions could contribute to the exaggerated inflammatory response seen in CF lung disease.
doi_str_mv 10.1016/j.jcf.2017.03.011
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We hypothesized that alternatively activated M2 macrophages are abnormal in CF lung disease. Methods Blood samples were collected from adults (n = 13) children (n = 27) with CF on admission for acute pulmonary exacerbation and when clinically stable. Monocytes were differentiated into macrophages and polarized into classical (M1) and alternatively-activated (M2) phenotypes, function determined ex-vivo and compared with healthy controls. Results In the absence of functional cystic fibrosis trans-membrane conductance regulator (CFTR), either naturally in patients with CF or induced with CFTR inhibitors, monocyte-derived macrophages do not respond to IL-13/IL-4, fail to polarize into M2s associated with a post-transcriptional failure to produce and express IL-13Rα1 on the macrophage surface Polarization to the M1 phenotype was unaffected. Conclusions CFTR-dependent imbalance of macrophage phenotypes and functions could contribute to the exaggerated inflammatory response seen in CF lung disease.</description><identifier>ISSN: 1569-1993</identifier><identifier>EISSN: 1873-5010</identifier><identifier>DOI: 10.1016/j.jcf.2017.03.011</identifier><identifier>PMID: 28428011</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adult ; Alternatively activated macrophages ; CFTR ; CFTR inhibitors ; Child ; Classically activated macrophages ; Cystic fibrosis (CF) ; Cystic Fibrosis - immunology ; Cystic Fibrosis - pathology ; Cystic Fibrosis - physiopathology ; Cystic Fibrosis Transmembrane Conductance Regulator - genetics ; Endocytosis ; Female ; Humans ; IL-13 receptor ; Inflammation - immunology ; Interleukin-13 - immunology ; Interleukin-13 Receptor alpha1 Subunit - immunology ; Interleukin-4 - immunology ; Lung - immunology ; Lung - pathology ; Lung - physiopathology ; Macrophage Activation - immunology ; Macrophages - immunology ; Macrophages - pathology ; Male ; Monocyte-derived macrophages ; Phagocytosis ; Pulmonary/Respiratory</subject><ispartof>Journal of cystic fibrosis, 2017-07, Vol.16 (4), p.475-482</ispartof><rights>European Cystic Fibrosis Society.</rights><rights>2017 European Cystic Fibrosis Society</rights><rights>Copyright © 2017 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-87f9c2717fb164f67be51273e4c18284dafef26d6e202b8cd5827ce26b5098793</citedby><cites>FETCH-LOGICAL-c451t-87f9c2717fb164f67be51273e4c18284dafef26d6e202b8cd5827ce26b5098793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28428011$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tarique, Abdullah A</creatorcontrib><creatorcontrib>Sly, Peter D</creatorcontrib><creatorcontrib>Holt, Patrick G</creatorcontrib><creatorcontrib>Bosco, Anthony</creatorcontrib><creatorcontrib>Ware, Robert S</creatorcontrib><creatorcontrib>Logan, Jayden</creatorcontrib><creatorcontrib>Bell, Scott C</creatorcontrib><creatorcontrib>Wainwright, Claire E</creatorcontrib><creatorcontrib>Fantino, Emmanuelle</creatorcontrib><title>CFTR-dependent defect in alternatively-activated macrophages in cystic fibrosis</title><title>Journal of cystic fibrosis</title><addtitle>J Cyst Fibros</addtitle><description>Abstract Background The role of the macrophages in cystic fibrosis (CF) lung disease has been poorly studied. We hypothesized that alternatively activated M2 macrophages are abnormal in CF lung disease. Methods Blood samples were collected from adults (n = 13) children (n = 27) with CF on admission for acute pulmonary exacerbation and when clinically stable. Monocytes were differentiated into macrophages and polarized into classical (M1) and alternatively-activated (M2) phenotypes, function determined ex-vivo and compared with healthy controls. Results In the absence of functional cystic fibrosis trans-membrane conductance regulator (CFTR), either naturally in patients with CF or induced with CFTR inhibitors, monocyte-derived macrophages do not respond to IL-13/IL-4, fail to polarize into M2s associated with a post-transcriptional failure to produce and express IL-13Rα1 on the macrophage surface Polarization to the M1 phenotype was unaffected. Conclusions CFTR-dependent imbalance of macrophage phenotypes and functions could contribute to the exaggerated inflammatory response seen in CF lung disease.</description><subject>Adult</subject><subject>Alternatively activated macrophages</subject><subject>CFTR</subject><subject>CFTR inhibitors</subject><subject>Child</subject><subject>Classically activated macrophages</subject><subject>Cystic fibrosis (CF)</subject><subject>Cystic Fibrosis - immunology</subject><subject>Cystic Fibrosis - pathology</subject><subject>Cystic Fibrosis - physiopathology</subject><subject>Cystic Fibrosis Transmembrane Conductance Regulator - genetics</subject><subject>Endocytosis</subject><subject>Female</subject><subject>Humans</subject><subject>IL-13 receptor</subject><subject>Inflammation - immunology</subject><subject>Interleukin-13 - immunology</subject><subject>Interleukin-13 Receptor alpha1 Subunit - immunology</subject><subject>Interleukin-4 - immunology</subject><subject>Lung - immunology</subject><subject>Lung - pathology</subject><subject>Lung - physiopathology</subject><subject>Macrophage Activation - immunology</subject><subject>Macrophages - immunology</subject><subject>Macrophages - pathology</subject><subject>Male</subject><subject>Monocyte-derived macrophages</subject><subject>Phagocytosis</subject><subject>Pulmonary/Respiratory</subject><issn>1569-1993</issn><issn>1873-5010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp9kUtLxDAQx4Movj-AF-nRS2smfSRFEGTxBYLg4xzSZKKp3XZNugv77U1Z9eDB08zhN39mfkPICdAMKFTnbdZqmzEKPKN5RgG2yD4InqclBbod-7KqU6jrfI8chNDSCFIudskeEwUTkd8nj7Obl6fU4AJ7g_2YGLSox8T1iepG9L0a3Qq7dap0bNSIJpkr7YfFu3rDMGF6HUanE-saPwQXjsiOVV3A4-96SF5vrl9md-nD4-397Ooh1UUJYyq4rTXjwG0DVWEr3mAJjOdYaBBxO6MsWlaZChlljdCmFIxrZFVT0lrwOj8kZ5vchR8-lxhGOXdBY9epHodlkCBqABajIKKwQePeIXi0cuHdXPm1BConj7KV0aOcPEqay-glzpx-xy-bOZrfiR9xEbjYABiPXDn0MmiHvUbjfBQozeD-jb_8M6071zutug9cY2iHZTTfxStkYJLK5-mR0x-B55TWBcu_ACB2l5c</recordid><startdate>20170701</startdate><enddate>20170701</enddate><creator>Tarique, Abdullah A</creator><creator>Sly, Peter D</creator><creator>Holt, Patrick G</creator><creator>Bosco, Anthony</creator><creator>Ware, Robert S</creator><creator>Logan, Jayden</creator><creator>Bell, Scott C</creator><creator>Wainwright, Claire E</creator><creator>Fantino, Emmanuelle</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20170701</creationdate><title>CFTR-dependent defect in alternatively-activated macrophages in cystic fibrosis</title><author>Tarique, Abdullah A ; Sly, Peter D ; Holt, Patrick G ; Bosco, Anthony ; Ware, Robert S ; Logan, Jayden ; Bell, Scott C ; Wainwright, Claire E ; Fantino, Emmanuelle</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-87f9c2717fb164f67be51273e4c18284dafef26d6e202b8cd5827ce26b5098793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adult</topic><topic>Alternatively activated macrophages</topic><topic>CFTR</topic><topic>CFTR inhibitors</topic><topic>Child</topic><topic>Classically activated macrophages</topic><topic>Cystic fibrosis (CF)</topic><topic>Cystic Fibrosis - immunology</topic><topic>Cystic Fibrosis - pathology</topic><topic>Cystic Fibrosis - physiopathology</topic><topic>Cystic Fibrosis Transmembrane Conductance Regulator - genetics</topic><topic>Endocytosis</topic><topic>Female</topic><topic>Humans</topic><topic>IL-13 receptor</topic><topic>Inflammation - immunology</topic><topic>Interleukin-13 - immunology</topic><topic>Interleukin-13 Receptor alpha1 Subunit - immunology</topic><topic>Interleukin-4 - immunology</topic><topic>Lung - immunology</topic><topic>Lung - pathology</topic><topic>Lung - physiopathology</topic><topic>Macrophage Activation - immunology</topic><topic>Macrophages - immunology</topic><topic>Macrophages - pathology</topic><topic>Male</topic><topic>Monocyte-derived macrophages</topic><topic>Phagocytosis</topic><topic>Pulmonary/Respiratory</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tarique, Abdullah A</creatorcontrib><creatorcontrib>Sly, Peter D</creatorcontrib><creatorcontrib>Holt, Patrick G</creatorcontrib><creatorcontrib>Bosco, Anthony</creatorcontrib><creatorcontrib>Ware, Robert S</creatorcontrib><creatorcontrib>Logan, Jayden</creatorcontrib><creatorcontrib>Bell, Scott C</creatorcontrib><creatorcontrib>Wainwright, Claire E</creatorcontrib><creatorcontrib>Fantino, Emmanuelle</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cystic fibrosis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tarique, Abdullah A</au><au>Sly, Peter D</au><au>Holt, Patrick G</au><au>Bosco, Anthony</au><au>Ware, Robert S</au><au>Logan, Jayden</au><au>Bell, Scott C</au><au>Wainwright, Claire E</au><au>Fantino, Emmanuelle</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CFTR-dependent defect in alternatively-activated macrophages in cystic fibrosis</atitle><jtitle>Journal of cystic fibrosis</jtitle><addtitle>J Cyst Fibros</addtitle><date>2017-07-01</date><risdate>2017</risdate><volume>16</volume><issue>4</issue><spage>475</spage><epage>482</epage><pages>475-482</pages><issn>1569-1993</issn><eissn>1873-5010</eissn><abstract>Abstract Background The role of the macrophages in cystic fibrosis (CF) lung disease has been poorly studied. We hypothesized that alternatively activated M2 macrophages are abnormal in CF lung disease. Methods Blood samples were collected from adults (n = 13) children (n = 27) with CF on admission for acute pulmonary exacerbation and when clinically stable. Monocytes were differentiated into macrophages and polarized into classical (M1) and alternatively-activated (M2) phenotypes, function determined ex-vivo and compared with healthy controls. Results In the absence of functional cystic fibrosis trans-membrane conductance regulator (CFTR), either naturally in patients with CF or induced with CFTR inhibitors, monocyte-derived macrophages do not respond to IL-13/IL-4, fail to polarize into M2s associated with a post-transcriptional failure to produce and express IL-13Rα1 on the macrophage surface Polarization to the M1 phenotype was unaffected. 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subjects Adult
Alternatively activated macrophages
CFTR
CFTR inhibitors
Child
Classically activated macrophages
Cystic fibrosis (CF)
Cystic Fibrosis - immunology
Cystic Fibrosis - pathology
Cystic Fibrosis - physiopathology
Cystic Fibrosis Transmembrane Conductance Regulator - genetics
Endocytosis
Female
Humans
IL-13 receptor
Inflammation - immunology
Interleukin-13 - immunology
Interleukin-13 Receptor alpha1 Subunit - immunology
Interleukin-4 - immunology
Lung - immunology
Lung - pathology
Lung - physiopathology
Macrophage Activation - immunology
Macrophages - immunology
Macrophages - pathology
Male
Monocyte-derived macrophages
Phagocytosis
Pulmonary/Respiratory
title CFTR-dependent defect in alternatively-activated macrophages in cystic fibrosis
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