Production of hexanal from hydrolyzed sunflower oil by lipoxygenase and hydroperoxid lyase enzymes

Hexanal was produced from hydrolyzed sunflower oil in two steps: 1) 13-hydroperoxy-9-(Z),11(E)-octadecadienoic acid (13-HPOD) was formed from linoleic acid (100 mM) by soybean lipoxygenase-1 isoenzyme (Lox-1) with O^sub 2^, the reaction resulted 68.7 mM 13-HPOD with a yield of 72%. 2) 13-HPOD (15 mM...

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Bibliographic Details
Published in:Biotechnology letters 2002-10, Vol.24 (20), p.1673-1675
Main Authors: MARCZY, Judit Sz, NEMETH, Agnes Sz, SAMU, Zsuzsanna, HAGER-VERESS, Adam, SZAJANI, Béla
Format: Article
Language:English
Subjects:
Acids
Biological and medical sciences
Biotechnology
Distillation
Enzyme engineering
Enzymes
Fundamental and applied biological sciences. Psychology
Hexanal
Hydrology
hydroperoxide lyase
Methods. Procedures. Technologies
Miscellaneous
Soybeans
Sunflower oil
Vegetable oils
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Summary:Hexanal was produced from hydrolyzed sunflower oil in two steps: 1) 13-hydroperoxy-9-(Z),11(E)-octadecadienoic acid (13-HPOD) was formed from linoleic acid (100 mM) by soybean lipoxygenase-1 isoenzyme (Lox-1) with O^sub 2^, the reaction resulted 68.7 mM 13-HPOD with a yield of 72%. 2) 13-HPOD (15 mM) was cleaved by spinach leaf hydroperoxide lyase resulting 8.2 mM hexanal (54% yield). Hexanal was isolated from the reaction mixture by repeated steam distillation.[PUBLICATION ABSTRACT]
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1020657618363