Healing activity evaluation of the galactomannan film obtained from Cassia grandis seeds with immobilized Cratylia mollis seed lectin

• Published in: International journal of biological macromolecules 2017-09, Vol.102, p.749-757
• Main Authors: Albuquerque, Priscilla B.S., Soares, Paulo A.G., Aragão-Neto, Adelmo C., Albuquerque, Giwellington S., Silva, Luís C.N., Lima-Ribeiro, Maria H.M., Silva Neto, Jacinto C., Coelho, Luana C.B.B., Correia, Maria T.S., Teixeira, José A.C., Carneiro-da-Cunha, Maria G.
• Format: Article
• Language: English
• Subjects: Animals; Cassia - chemistry; Cell Proliferation - drug effects; Epithelial Cells - cytology; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Male; Mannans - chemistry; Mannans - pharmacology; Natural polymers; Plant Lectins - chemistry; Proliferating Cell Nuclear Antigen - metabolism; Rats; Seeds - chemistry; Topical wound curatives; Wound healing; Wound Healing - drug effects
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2017.04.064

[Display omitted] •Cramoll 1,4 was efficiently immobilized in films based on the galactomannan extracted from C. grandis seeds.•Cramoll 1,4 maintained 90.94% of the initial lectin hemagglutinating activity after the immobilization process.•Galactomannan film and the film with immobilized Cramoll 1.4 showed no cytotoxicity.•Galactomannan film with immobilized Cramoll 1,4 completely closed the wounds with 11days of experiment.•Galactomannan film and the film with immobilized Cramoll 1.4 showed to be a promising candidate as topical wound curatives. Galactomannan films from Cassia grandis seeds, associated or not with Cramoll 1,4, were used on topical wounds of rats for the evaluation of the healing process during 14days. All of the films were evaluated by cytotoxic assay, FTIR and lectin hemagglutinating activity (HA). Forty-five male rats were submitted to aseptic dermal wounds (Ø=0.8cm) and divided in groups (n=15): control, test 1, and test 2, treated respectively with saline, galactomannan film and film with immobilized Cramoll 1,4. Macroscopic evaluations were performed by clinical observations and area measurements, and microscopic analysis by histological criteria. Epithelial cell proliferation and differentiation was immunohistochemically assessed using CK14 and PCNA. The presence of CO peaks in the FTIR spectrum confirmed the immobilization of Cramoll 1,4 in the film, while the residual HA confirmed the stability of the lectin after immobilization with 90.94% of the initial HA. The films presented non-cytotoxicity and cell viability exceeding 80%. All of the animals presented re-epithelization around 10days, furthermore test 2 group showed a diffuse response at the stromal tissue and the basal layer associated with wounds completely closed with 11days of experiment. The results suggest a promising use of the films as topical wound curatives.