A highly regular fucosylated chondroitin sulfate from the sea cucumber Massinium magnum: Structure and effects on coagulation

•The structure of the natural fucosylated chondroitin sulfate (FCS) was determined.•The sea cucumber Massinium magnum was used as a source of FCS.•The backbone of the polysaccharide consists mainly of chondroitin sulfate E units.•The only one type of Fuc3S4S branches attached to O-3 of GlcA was foun...

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Bibliographic Details
Published in:Carbohydrate polymers 2017-07, Vol.167, p.20-26
Main Authors: Ustyuzhanina, Nadezhda E., Bilan, Maria I., Dmitrenok, Andrey S., Borodina, Elizaveta Yu, Stonik, Valentin A., Nifantiev, Nikolay E., Usov, Anatolii I.
Format: Article
Language:English
Subjects:
Animals
Anticoagulant
Blood Coagulation - drug effects
Chondroitin Sulfates - chemistry
Fucose
Fucosylated chondroitin sulfate
Humans
Massinium magnum
Platelet Aggregation - drug effects
Sea cucumber
Sea Cucumbers - chemistry
Structure
Thrombin Time
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Summary:•The structure of the natural fucosylated chondroitin sulfate (FCS) was determined.•The sea cucumber Massinium magnum was used as a source of FCS.•The backbone of the polysaccharide consists mainly of chondroitin sulfate E units.•The only one type of Fuc3S4S branches attached to O-3 of GlcA was found.•Anticoagulant and antithrombotic properties of FCS were studied in vitro. A fucosylated chondroitin sulfate MM was isolated from the sea cucumber Massinium magnum. Structure of this polysaccharide was determined using chemical and NMR spectroscopic methods. The backbone of MM was shown to consist mainly of chondroitin sulfate E units with a small portion (about 10%) of chondroitin sulfate A fragments. Practically one type of branches Fuc3S4S attached to O-3 of GlcA residues was found in the polysaccharide molecules. The main repeating units of MM are →4)-[α-l-Fuc3S4S-(1→3)]-β-d-GlcA-(1→3)-β-d-GalNAc4S6S-(1→, whereas the minor repeating units are →4)-[α-l-Fuc3S4S-(1→3)]-β-d-GlcA-(1→3)-β-d-GalNAc4S-(1→. Anticoagulant activity of MM determined in APTT and TT tests was shown to be lower than that of heparin, but higher than that of enoxaparin. In the experiments with purified proteins MM effectively potentiated inhibition of thrombin and factor Xa by ATIII. Besides, MM did not induce platelets aggregation in platelets rich plasma.
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2017.02.101