Down-regulation of Pin1 in Temporal Lobe Epilepsy Patients and Mouse Model

Peptidyl-prolyl cis – trans isomerase NIMA-interacting 1 (Pin1) is a unique PPIase belonging to the parvulin family, and it isomerizes peptide bond between phospho-(Ser/Thr) and Pro. Pin1 has been linked to the pathogenesis of various human diseases; however, its exact biological functions remain un...

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Bibliographic Details
Published in:Neurochemical research 2017-04, Vol.42 (4), p.1211-1218
Main Authors: Tang, Lan, Zhang, Yanke, Chen, Guojun, Xiong, Yan, Wang, Xuefeng, Zhu, Binglin
Format: Article
Language:English
Subjects:
Adolescent
Adult
Animals
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cell Biology
Child
Disease Models, Animal
Down-Regulation - drug effects
Down-Regulation - physiology
Epilepsy, Temporal Lobe - chemically induced
Epilepsy, Temporal Lobe - metabolism
Epilepsy, Temporal Lobe - pathology
Hippocampus - drug effects
Hippocampus - metabolism
Hippocampus - pathology
Humans
Male
Mice
Mice, Inbred C57BL
Middle Aged
Neocortex - drug effects
Neocortex - metabolism
Neocortex - pathology
Neurochemistry
Neurology
Neurosciences
NIMA-Interacting Peptidylprolyl Isomerase - metabolism
Original Paper
Pilocarpine - toxicity
Young Adult
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Summary:Peptidyl-prolyl cis – trans isomerase NIMA-interacting 1 (Pin1) is a unique PPIase belonging to the parvulin family, and it isomerizes peptide bond between phospho-(Ser/Thr) and Pro. Pin1 has been linked to the pathogenesis of various human diseases; however, its exact biological functions remain unclear. The aim of the present study is to explore the expression pattern of Pin1 in patients with refractory epilepsy and in a chronic pilocarpine-induced epileptic mouse model. Using Western blot, immunofluorescence and immunoprecipitation analysis, we found that Pin1 protein was mainly distributed in neurons, demonstrated by colocalization with the dendritic marker, MAP2. However, the expression of Pin1 decreased remarkably in epileptic patients and experimental mice. Furthermore, the reciprocal coimmunoprecipitation analysis showed that Pin1 interacted with NR2A and NR2B-containing NMDA receptors not AMPA receptors in epileptic mouse models. Our results are the first to indicate that the expression of Pin1 in epileptic brain tissue could play important roles in epilepsy.
ISSN:0364-3190
1573-6903
DOI:10.1007/s11064-016-2158-8