Human papillomavirus (HPV) infection in a case‐control study of oral squamous cell carcinoma and its increasing trend in northeastern Thailand

Human papillomavirus (HPV) is an independent risk factor for development of oral squamous cell carcinoma (OSCC). This study aimed to investigate the role of HPV infection and the trend in percentage of HPV‐associated OSCC over a 5‐year period in northeastern Thailand. In this case‐control study, 91...

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Bibliographic Details
Published in:Journal of medical virology 2017-06, Vol.89 (6), p.1096-1101
Main Authors: Phusingha, Pensiri, Ekalaksananan, Tipaya, Vatanasapt, Patravoot, Loyha, Kulchaya, Promthet, Supannee, Kongyingyoes, Bunkerd, Patarapadungkit, Natcha, Chuerduangphui, Jureeporn, Pientong, Chamsai
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Carcinoma, Squamous Cell - epidemiology
Carcinoma, Squamous Cell - virology
Case-Control Studies
DNA extraction
DNA, Viral - genetics
Female
Gene Expression Profiling
Genotype
Genotyping Techniques
Human papillomavirus
Humans
In Situ Hybridization
Infections
Male
Middle Aged
Mouth Neoplasms - epidemiology
Mouth Neoplasms - virology
Oncogene Proteins, Viral - biosynthesis
oncogenes
Papillomaviridae
Papillomaviridae - classification
Papillomaviridae - genetics
Papillomaviridae - isolation & purification
Papillomavirus Infections - complications
Papillomavirus Infections - epidemiology
Polymerase Chain Reaction
RNA extraction
Studies
Thailand - epidemiology
Transcription, Genetic
Trends
Virology
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Summary:Human papillomavirus (HPV) is an independent risk factor for development of oral squamous cell carcinoma (OSCC). This study aimed to investigate the role of HPV infection and the trend in percentage of HPV‐associated OSCC over a 5‐year period in northeastern Thailand. In this case‐control study, 91 exfoliated oral cell samples and 80 lesion cell samples from OSCC cases and exfoliated oral cells from 100 age/gender‐matched controls were collected. HPV infection was investigated by PCR using GP5+/GP6+ primers followed by HPV genotyping using reverse line blot hybridization. Quantitative RT‐PCR was used to evaluate HPV oncogene transcription. Temporal trends of HPV infection were evaluated in archived formalin‐fixed paraffin‐embedded (FFPE) OSCC tissues using in situ hybridization. HPV DNA was found in 17.5% (14/80) of lesion samples from OSCC cases and 29.7% (27/91) of exfoliated oral cell samples from the same cases. These values were significantly higher than in exfoliated oral cell samples from controls (13%, 13/100). HPV‐16 was the genotype most frequently found in OSCC cases (92.8%, 13/14 infected cases). Interestingly, HPV oncogene mRNA expression was detected and correlated with OSCC cases (P 
ISSN:0146-6615
1096-9071
DOI:10.1002/jmv.24744