Loading…

Characterization of bacterial communities from Masseiras, a unique Portuguese greenhouse agricultural system

“Masseiras” is an ancient Portuguese agriculture system, where soil was developed from sand dunes enriched with seaweeds over more than a century. Due to the importance for the local economy, this system evolved for greenhouse structures. In this study we compared the bacterial community composition...

Full description

Saved in:
Bibliographic Details
Published in:Antonie van Leeuwenhoek 2017-05, Vol.110 (5), p.665-676
Main Authors: Becerra-Castro, Cristina, Lopes, Ana Rita, Teixeira, Sara, Silva, M. Elisabete F., Pimenta, Elisabete, Manaia, Célia M., Nunes, Olga C.
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c432t-6b6f001c372a6da13e5d354c3ceacb780abe351f2df8d065e8812e5457bb80983
cites cdi_FETCH-LOGICAL-c432t-6b6f001c372a6da13e5d354c3ceacb780abe351f2df8d065e8812e5457bb80983
container_end_page 676
container_issue 5
container_start_page 665
container_title Antonie van Leeuwenhoek
container_volume 110
creator Becerra-Castro, Cristina
Lopes, Ana Rita
Teixeira, Sara
Silva, M. Elisabete F.
Pimenta, Elisabete
Manaia, Célia M.
Nunes, Olga C.
description “Masseiras” is an ancient Portuguese agriculture system, where soil was developed from sand dunes enriched with seaweeds over more than a century. Due to the importance for the local economy, this system evolved for greenhouse structures. In this study we compared the bacterial community composition and structure of “Masseiras” soil, aiming at assessing the potential impact of different agricultural practices. The bulk soil of two greenhouses (following or not the recommended agriculture good practices, FGP and NFGP, respectively) was compared based on their physicochemical properties and bacterial community. In both FGP and NFGP, Proteobacteria , Acidobacteria, Actinobacteria, Bacteroidetes, Firmicutes , and Gemmatimonadetes were in a proportion of 5:1:1:1:1:1. However, the bacterial community of soil FGP was richer and more diverse than that of soil NFGP. Members of the classes Bacilli and Gemm-1, with higher relative abundance in NFGP and FGP, respectively, were those contributing most for distinguishing the bacterial communities of both soils. The differences in the structure of the bacterial communities correlated (Mantel test) with some soil physicochemical properties, such as electrical conductivity and nitrate and Zn contents, which were significantly higher in soil NFGP than in soil FGP.
doi_str_mv 10.1007/s10482-017-0833-7
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1891884971</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>4321601069</sourcerecordid><originalsourceid>FETCH-LOGICAL-c432t-6b6f001c372a6da13e5d354c3ceacb780abe351f2df8d065e8812e5457bb80983</originalsourceid><addsrcrecordid>eNqNkUtr3TAQRkVpaW4eP6CbIOimi7gZSdbDy3Bp0kJCs2jXQpbHNw62lUjWIvn11cVpKYVAV3rMmU8jDiEfGHxmAPo8MagNr4DpCowQlX5DNkxqXjWqad6SDQCISoHmB-QwpftybJTR78kBN0wCSL0h4_bORecXjMOzW4Yw09DTdr1wI_VhmvI8LAMm2scw0RuXEg7RpTPqaKk8ZqS3IS55lzEh3UXE-S7ksnW7OPg8LjmWnPSUFpyOybvejQlPXtYj8vPyy4_t1-r6-9W37cV15WvBl0q1qgdgXmjuVOeYQNkJWXvh0flWG3AtCsl63vWmAyXRGMZR1lK3rYHGiCPyac19iKEMmBY7DcnjOLoZy2yWmYYZUzea_QeqpKyBy33qx3_Q-5DjXD5SKKM46MaoQrGV8jGkFLG3D3GYXHyyDOzeml2t2WLN7q1ZXXpOX5JzO2H3p-O3pgLwFUilNO8w_vX0q6m_AF1io2I</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1886207986</pqid></control><display><type>article</type><title>Characterization of bacterial communities from Masseiras, a unique Portuguese greenhouse agricultural system</title><source>Springer Link</source><creator>Becerra-Castro, Cristina ; Lopes, Ana Rita ; Teixeira, Sara ; Silva, M. Elisabete F. ; Pimenta, Elisabete ; Manaia, Célia M. ; Nunes, Olga C.</creator><creatorcontrib>Becerra-Castro, Cristina ; Lopes, Ana Rita ; Teixeira, Sara ; Silva, M. Elisabete F. ; Pimenta, Elisabete ; Manaia, Célia M. ; Nunes, Olga C.</creatorcontrib><description>“Masseiras” is an ancient Portuguese agriculture system, where soil was developed from sand dunes enriched with seaweeds over more than a century. Due to the importance for the local economy, this system evolved for greenhouse structures. In this study we compared the bacterial community composition and structure of “Masseiras” soil, aiming at assessing the potential impact of different agricultural practices. The bulk soil of two greenhouses (following or not the recommended agriculture good practices, FGP and NFGP, respectively) was compared based on their physicochemical properties and bacterial community. In both FGP and NFGP, Proteobacteria , Acidobacteria, Actinobacteria, Bacteroidetes, Firmicutes , and Gemmatimonadetes were in a proportion of 5:1:1:1:1:1. However, the bacterial community of soil FGP was richer and more diverse than that of soil NFGP. Members of the classes Bacilli and Gemm-1, with higher relative abundance in NFGP and FGP, respectively, were those contributing most for distinguishing the bacterial communities of both soils. The differences in the structure of the bacterial communities correlated (Mantel test) with some soil physicochemical properties, such as electrical conductivity and nitrate and Zn contents, which were significantly higher in soil NFGP than in soil FGP.</description><identifier>ISSN: 0003-6072</identifier><identifier>EISSN: 1572-9699</identifier><identifier>DOI: 10.1007/s10482-017-0833-7</identifier><identifier>PMID: 28150057</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Actinobacteria ; Agricultural practices ; Agriculture ; Algae ; Bacteria ; Bacteria - classification ; Bacteria - isolation &amp; purification ; Biomedical and Life Sciences ; Biota ; Chemical Phenomena ; Community composition ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Dunes ; Farm buildings ; Farming systems ; Firmicutes ; Greenhouses ; Horticulture ; Life Sciences ; Local economy ; Medical Microbiology ; Microbiology ; Original Paper ; Physicochemical properties ; Plant Sciences ; Portugal ; Proteobacteria ; Relative abundance ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Soil - chemistry ; Soil Microbiology ; Soil microorganisms ; Soil properties ; Soil Science &amp; Conservation</subject><ispartof>Antonie van Leeuwenhoek, 2017-05, Vol.110 (5), p.665-676</ispartof><rights>Springer International Publishing Switzerland 2017</rights><rights>Antonie van Leeuwenhoek is a copyright of Springer, 2017.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c432t-6b6f001c372a6da13e5d354c3ceacb780abe351f2df8d065e8812e5457bb80983</citedby><cites>FETCH-LOGICAL-c432t-6b6f001c372a6da13e5d354c3ceacb780abe351f2df8d065e8812e5457bb80983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28150057$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Becerra-Castro, Cristina</creatorcontrib><creatorcontrib>Lopes, Ana Rita</creatorcontrib><creatorcontrib>Teixeira, Sara</creatorcontrib><creatorcontrib>Silva, M. Elisabete F.</creatorcontrib><creatorcontrib>Pimenta, Elisabete</creatorcontrib><creatorcontrib>Manaia, Célia M.</creatorcontrib><creatorcontrib>Nunes, Olga C.</creatorcontrib><title>Characterization of bacterial communities from Masseiras, a unique Portuguese greenhouse agricultural system</title><title>Antonie van Leeuwenhoek</title><addtitle>Antonie van Leeuwenhoek</addtitle><addtitle>Antonie Van Leeuwenhoek</addtitle><description>“Masseiras” is an ancient Portuguese agriculture system, where soil was developed from sand dunes enriched with seaweeds over more than a century. Due to the importance for the local economy, this system evolved for greenhouse structures. In this study we compared the bacterial community composition and structure of “Masseiras” soil, aiming at assessing the potential impact of different agricultural practices. The bulk soil of two greenhouses (following or not the recommended agriculture good practices, FGP and NFGP, respectively) was compared based on their physicochemical properties and bacterial community. In both FGP and NFGP, Proteobacteria , Acidobacteria, Actinobacteria, Bacteroidetes, Firmicutes , and Gemmatimonadetes were in a proportion of 5:1:1:1:1:1. However, the bacterial community of soil FGP was richer and more diverse than that of soil NFGP. Members of the classes Bacilli and Gemm-1, with higher relative abundance in NFGP and FGP, respectively, were those contributing most for distinguishing the bacterial communities of both soils. The differences in the structure of the bacterial communities correlated (Mantel test) with some soil physicochemical properties, such as electrical conductivity and nitrate and Zn contents, which were significantly higher in soil NFGP than in soil FGP.</description><subject>Actinobacteria</subject><subject>Agricultural practices</subject><subject>Agriculture</subject><subject>Algae</subject><subject>Bacteria</subject><subject>Bacteria - classification</subject><subject>Bacteria - isolation &amp; purification</subject><subject>Biomedical and Life Sciences</subject><subject>Biota</subject><subject>Chemical Phenomena</subject><subject>Community composition</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>Dunes</subject><subject>Farm buildings</subject><subject>Farming systems</subject><subject>Firmicutes</subject><subject>Greenhouses</subject><subject>Horticulture</subject><subject>Life Sciences</subject><subject>Local economy</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Original Paper</subject><subject>Physicochemical properties</subject><subject>Plant Sciences</subject><subject>Portugal</subject><subject>Proteobacteria</subject><subject>Relative abundance</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Soil - chemistry</subject><subject>Soil Microbiology</subject><subject>Soil microorganisms</subject><subject>Soil properties</subject><subject>Soil Science &amp; Conservation</subject><issn>0003-6072</issn><issn>1572-9699</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNqNkUtr3TAQRkVpaW4eP6CbIOimi7gZSdbDy3Bp0kJCs2jXQpbHNw62lUjWIvn11cVpKYVAV3rMmU8jDiEfGHxmAPo8MagNr4DpCowQlX5DNkxqXjWqad6SDQCISoHmB-QwpftybJTR78kBN0wCSL0h4_bORecXjMOzW4Yw09DTdr1wI_VhmvI8LAMm2scw0RuXEg7RpTPqaKk8ZqS3IS55lzEh3UXE-S7ksnW7OPg8LjmWnPSUFpyOybvejQlPXtYj8vPyy4_t1-r6-9W37cV15WvBl0q1qgdgXmjuVOeYQNkJWXvh0flWG3AtCsl63vWmAyXRGMZR1lK3rYHGiCPyac19iKEMmBY7DcnjOLoZy2yWmYYZUzea_QeqpKyBy33qx3_Q-5DjXD5SKKM46MaoQrGV8jGkFLG3D3GYXHyyDOzeml2t2WLN7q1ZXXpOX5JzO2H3p-O3pgLwFUilNO8w_vX0q6m_AF1io2I</recordid><startdate>20170501</startdate><enddate>20170501</enddate><creator>Becerra-Castro, Cristina</creator><creator>Lopes, Ana Rita</creator><creator>Teixeira, Sara</creator><creator>Silva, M. Elisabete F.</creator><creator>Pimenta, Elisabete</creator><creator>Manaia, Célia M.</creator><creator>Nunes, Olga C.</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20170501</creationdate><title>Characterization of bacterial communities from Masseiras, a unique Portuguese greenhouse agricultural system</title><author>Becerra-Castro, Cristina ; Lopes, Ana Rita ; Teixeira, Sara ; Silva, M. Elisabete F. ; Pimenta, Elisabete ; Manaia, Célia M. ; Nunes, Olga C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-6b6f001c372a6da13e5d354c3ceacb780abe351f2df8d065e8812e5457bb80983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Actinobacteria</topic><topic>Agricultural practices</topic><topic>Agriculture</topic><topic>Algae</topic><topic>Bacteria</topic><topic>Bacteria - classification</topic><topic>Bacteria - isolation &amp; purification</topic><topic>Biomedical and Life Sciences</topic><topic>Biota</topic><topic>Chemical Phenomena</topic><topic>Community composition</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>Dunes</topic><topic>Farm buildings</topic><topic>Farming systems</topic><topic>Firmicutes</topic><topic>Greenhouses</topic><topic>Horticulture</topic><topic>Life Sciences</topic><topic>Local economy</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Original Paper</topic><topic>Physicochemical properties</topic><topic>Plant Sciences</topic><topic>Portugal</topic><topic>Proteobacteria</topic><topic>Relative abundance</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Soil - chemistry</topic><topic>Soil Microbiology</topic><topic>Soil microorganisms</topic><topic>Soil properties</topic><topic>Soil Science &amp; Conservation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Becerra-Castro, Cristina</creatorcontrib><creatorcontrib>Lopes, Ana Rita</creatorcontrib><creatorcontrib>Teixeira, Sara</creatorcontrib><creatorcontrib>Silva, M. Elisabete F.</creatorcontrib><creatorcontrib>Pimenta, Elisabete</creatorcontrib><creatorcontrib>Manaia, Célia M.</creatorcontrib><creatorcontrib>Nunes, Olga C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Antonie van Leeuwenhoek</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Becerra-Castro, Cristina</au><au>Lopes, Ana Rita</au><au>Teixeira, Sara</au><au>Silva, M. Elisabete F.</au><au>Pimenta, Elisabete</au><au>Manaia, Célia M.</au><au>Nunes, Olga C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of bacterial communities from Masseiras, a unique Portuguese greenhouse agricultural system</atitle><jtitle>Antonie van Leeuwenhoek</jtitle><stitle>Antonie van Leeuwenhoek</stitle><addtitle>Antonie Van Leeuwenhoek</addtitle><date>2017-05-01</date><risdate>2017</risdate><volume>110</volume><issue>5</issue><spage>665</spage><epage>676</epage><pages>665-676</pages><issn>0003-6072</issn><eissn>1572-9699</eissn><abstract>“Masseiras” is an ancient Portuguese agriculture system, where soil was developed from sand dunes enriched with seaweeds over more than a century. Due to the importance for the local economy, this system evolved for greenhouse structures. In this study we compared the bacterial community composition and structure of “Masseiras” soil, aiming at assessing the potential impact of different agricultural practices. The bulk soil of two greenhouses (following or not the recommended agriculture good practices, FGP and NFGP, respectively) was compared based on their physicochemical properties and bacterial community. In both FGP and NFGP, Proteobacteria , Acidobacteria, Actinobacteria, Bacteroidetes, Firmicutes , and Gemmatimonadetes were in a proportion of 5:1:1:1:1:1. However, the bacterial community of soil FGP was richer and more diverse than that of soil NFGP. Members of the classes Bacilli and Gemm-1, with higher relative abundance in NFGP and FGP, respectively, were those contributing most for distinguishing the bacterial communities of both soils. The differences in the structure of the bacterial communities correlated (Mantel test) with some soil physicochemical properties, such as electrical conductivity and nitrate and Zn contents, which were significantly higher in soil NFGP than in soil FGP.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>28150057</pmid><doi>10.1007/s10482-017-0833-7</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0003-6072
ispartof Antonie van Leeuwenhoek, 2017-05, Vol.110 (5), p.665-676
issn 0003-6072
1572-9699
language eng
recordid cdi_proquest_miscellaneous_1891884971
source Springer Link
subjects Actinobacteria
Agricultural practices
Agriculture
Algae
Bacteria
Bacteria - classification
Bacteria - isolation & purification
Biomedical and Life Sciences
Biota
Chemical Phenomena
Community composition
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Ribosomal - chemistry
DNA, Ribosomal - genetics
Dunes
Farm buildings
Farming systems
Firmicutes
Greenhouses
Horticulture
Life Sciences
Local economy
Medical Microbiology
Microbiology
Original Paper
Physicochemical properties
Plant Sciences
Portugal
Proteobacteria
Relative abundance
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Soil - chemistry
Soil Microbiology
Soil microorganisms
Soil properties
Soil Science & Conservation
title Characterization of bacterial communities from Masseiras, a unique Portuguese greenhouse agricultural system
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T18%3A35%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Characterization%20of%20bacterial%20communities%20from%20Masseiras,%20a%20unique%20Portuguese%20greenhouse%20agricultural%20system&rft.jtitle=Antonie%20van%20Leeuwenhoek&rft.au=Becerra-Castro,%20Cristina&rft.date=2017-05-01&rft.volume=110&rft.issue=5&rft.spage=665&rft.epage=676&rft.pages=665-676&rft.issn=0003-6072&rft.eissn=1572-9699&rft_id=info:doi/10.1007/s10482-017-0833-7&rft_dat=%3Cproquest_cross%3E4321601069%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c432t-6b6f001c372a6da13e5d354c3ceacb780abe351f2df8d065e8812e5457bb80983%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1886207986&rft_id=info:pmid/28150057&rfr_iscdi=true