Deinococcus malanensis sp. nov., isolated from radiation-polluted soil

• Published in: Archives of microbiology 2017-05, Vol.199 (4), p.621-626
• Main Authors: Zhu, Jing, Li, Shan-Hui, Tang, Qi-Yong, Chu, Min, Wang, Wei, Salam, Nimaichand, Li, Li, Hozzein, Wael N., Zhang, Zhi-Dong, Li, Wen-Jun
• Format: Article
• Language: English
• Subjects: Biochemistry; Biomedical and Life Sciences; Biotechnology; Cell Biology; Deinococcus; Deinococcus - chemistry; Deinococcus - classification; Deinococcus - genetics; Deinococcus - isolation & purification; DNA, Bacterial - chemistry; Ecology; Fatty acids; Fatty Acids - analysis; Genotype; Laboratories; Life Sciences; Microbial Ecology; Microbiology; Microscopy; Original Paper; Phylogeny; Radiation; RNA, Ribosomal, 16S - genetics; Soil contamination; Soil Microbiology; Soil Pollutants, Radioactive; Soil pollution; Taxonomy
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-016-1335-0

A Gram-staining positive, non-spore forming, short rod-shaped and coccus-shaped, non-motile, pink-colored, gamma- and UV-resistant strain, designated T93 T was isolated from soil of Malan area in Xinjiang Uyghur Autonomous Region, Northwest China. The taxonomic position of the new isolate was determined using a polyphasic approach. Strain T93 T shared the highest 16 S rRNA gene sequence similarity with Deinococcus deserti VCD115 T (97.54%). The genomic DNA G+C content of the isolate T93 T was 61.7 mol%. The predominant menaquinone was MK-8, while the major cellular fatty acids were iso -C 16:0 , C 15:1 ω 6 c , C 16:0 , C 17:1 ω 8 c and Summed Feature 3 (comprising C 16:1 ω 7 c and/or C 16:1 ω 6 c ). The major polar lipid profiles consisted of diphosphatidylglycerol and phosphatidylinositol mannoside. Based on the phenotypic and genotypic data, strain T93 T is considered to represent a novel species of the genus Deinococcus , for which the name Deinococcus malanensis sp. nov. is proposed. The type strain is T93 T (= KCTC 33563 T  = JCM 30331 T ).